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Quality reagents are key to the accurate localization and identification of antigens on histological sections. We've learned this from the thousands of hours that we've spent validating antibodies in IHC.
Find the IHC kits and reagents below that you need to get clean, specific staining even when detecting low abundance markers. Alternatively, consult our IHC application guide or view our IHC protocols.
- Antigen retrieval
- Primary antibody dilution
- Directly conjugated primary antibodies
- Chromogenic signal amplification
- Fluorescent signal amplification
- Mouse antibodies on mouse tissue
Block endogenous peroxidases, if using HRP for detection, with hydrogen peroxide blocking reagent.
Block endogenous biotin, if using biotin-based amplification, with a biotin blocking agent.
Dilute primary antibodies for IHC using our validated antibody diluent ab64211.
Visualize primary antibody staining using a modern micro-polymer method and highly sensitive micro-polymer kits. Or select from HRP-polymer-conjugated secondary antibodies and chromogenic substrate kits.
Alternatively, use the more traditional streptavidin-biotin (LSAB ABC) method and complete IHC detection ABC kits. Or select from biotin-conjugated secondary antibodies, streptavidin-enzyme conjugates, and chromogenic substrate kits.
Stop high background caused by anti-mouse secondary antibodies binding to mouse tissues with a mouse-on-mouse kit.
Identify the location of antibody staining with chromogenic and fluorescent counterstains and special stains for specific cell types, proteins, carbohydrates, and metabolites.