Immunohistochemistry kits and reagents
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Get sharp and strong staining in your immunohistochemistry (IHC) with optimized reagents
Quality reagents are key to the accurate localization and identification of antigens on histological sections. We've learned this from the thousands of hours that we've spent validating antibodies in IHC.
Find the IHC kits and reagents below that you need to get clean, specific staining even when detecting low abundance markers. Alternatively, consult our IHC application guide or view our IHC protocols.
Contents
- Antigen retrieval
- Blocking
- Primary antibody dilution
- Directly conjugated primary antibodies
- Chromogenic signal amplification
- Fluorescent signal amplification
- Mouse antibodies on mouse tissue
- Counterstaining
- Mounting
Antigen retrieval
Use optimized buffers for heat-induced antigen retrieval and reagents for enzymatic antigen retrieval.
Blocking
Block non-specific antibody binding with normal serums optimized for blocking in IHC or use a convenient protein blocking buffer.
Block endogenous peroxidases, if using HRP for detection, with hydrogen peroxide blocking reagent.
Block endogenous biotin, if using biotin-based amplification, with a biotin blocking agent.
Primary antibody diluents
Dilute primary antibodies for IHC using our validated antibody diluent ab64211.
Directly conjugated primary antibodies
Use our directly conjugated primary antibodies to speed up and simplify the IHC protocol as they omit the need for a secondary antibody staining step. Our primary conjugates are recombinantly engineered to provide high specificity and batch-to-batch consistency.
If your antibody of choice is not available in a suitable conjugated format, you can use our antibody conjugation kits.
Chromogenic signal amplification
Visualize primary antibody staining using a modern micro-polymer method and highly sensitive micro-polymer kits. Or select from HRP-polymer-conjugated secondary antibodies and chromogenic substrate kits.
Alternatively, use the more traditional streptavidin-biotin (LSAB ABC) method and complete IHC detection ABC kits. Or select from biotin-conjugated secondary antibodies, streptavidin-enzyme conjugates, and chromogenic substrate kits.
If sensitivity is not an issue, then use an HRP conjugated secondary antibody, or a rapid HRP or AP antibody conjugation kit.
Fluorescent signal amplification
Use an Alexa-fluor® dye-conjugated secondary antibody or a rapid antibody-dye conjugation kit.
Mouse antibodies on mouse tissue
Stop high background caused by anti-mouse secondary antibodies binding to mouse tissues with a mouse-on-mouse kit.
Counterstaining
Identify the location of antibody staining with chromogenic and fluorescent counterstains and special stains for specific cell types, proteins, carbohydrates, and metabolites.
Mounting
Use aqueous mounting media for chromogenic IHC, or specialist mounting media for fluorescent IHC.