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新型コロナウイルス (COVID-19)に伴う当社の対応について

Antibody purification and concentration kits

Related

  • Overview of affinity purification
    • Antibody binding affinities of Protein A, Protein G, Protein L and jacalin
      • Antibody conjugation kits
        • BSA and Azide Free formulations
          • Low Endotoxin, Azide free antibodies

            Select the right kit for your antibody purification and concentration needs with our guide
            ​

            Overview

            • Select the right kit for your antibody purification and concentration 
            • Antibody purification kits
              • Purification of antibodies from unwanted buffer constituents (eg BSA)
              • Purification of antibodies from TCS
              • Purification of antibodies from serum or ascites fluid
              • Magnetic antibody purification system
            • Antibody concentration and clean-up kits 


            Select the right kit for your antibody purification and concentration

            Our easy-to-use flow diagrams will help you choose the best antibody purification kit or antibody concentration kit for your needs.

            Purification selection - kitsPurification selection - nanoparticles
            Select a kit that is compatible with our Lightning-Link®, Oligonucleotide, and metal conjugation kitsSelect a kit that is compatible with our nanoparticle conjugation kits

            Antibody purification kits

            ​Many commercially available and in-house manufactured antibodies often contain substances that may interfere with applications such as antibody conjugation, making purification a key consideration. Our antibody purification kits are suitable for purifying antibodies from serum, tissue culture supernatant (TCS), and ascites fluid. The resulting purified antibodies are fully compatible with our conjugation kits.

            Purification of antibodies from unwanted buffer constituents (eg, BSA)

            Antibodies are often stored in buffers that contain substances other than the antibody itself. Our antibody purification kits highlighted in Table 1 are designed to remove protein additives, leaving the antibody in a format suitable for transfer to a conjugation-friendly buffer.

            Table 1.

            Product nameUseConjugation kit compatibility 
            Antibody Purification Kit (Protein A) (ab102784)

            Suitable for purifying IgG from multiple species

            Removes contaminants including BSA, glycine, tris, and azide

            Purifies antibodies from crude samples such as ascites fluid or immune serum

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            Antibody Purification Kit (Protein G) (ab128747)

            Suitable for purifying IgG from multiple species

            Removes contaminants including BSA, glycine, tris, and azide

            Purifies antibodies from crude samples such as ascites fluid or immune serum

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits

            Mouse Antibody Purification Kit (ab128745)

            Suitable for purifying mouse IgG only

            Removes contaminants including BSA, glycine, tris, and azide

            Purifies antibodies from crude samples such as ascites fluid or immune serum

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            BSA Removal Kit (ab173231)

            Suitable for purifying any antibody sub-type and species

            Removes BSA, glycine, tris, and azide 

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            Antibody Purification Kit – Nanoparticles (ab204909)

            Suitable for purifying IgG from multiple species

            Removes contaminants including BSA, glycine, tris, and azide

            Purifies antibodies from crude samples such as ascites fluid or immune serum
            Nanoparticle conjugation kits
            Mouse Antibody Purification Kit – Nanoparticles (ab204910)

            Suitable for purifying mouse IgG only

            Removes contaminants including BSA, glycine, tris, and azide

            Purifies antibodies from crude samples such as ascites fluid or immune serum
            Nanoparticle conjugation kits
            BSA Removal Kit – Nanoparticles (ab204912)

            Suitable for purifying any antibody sub-type and species

            Removes BSA glycine, tris, and azide

            Nanoparticle conjugation kits

            The method involves capturing the antibody on agarose beads coupled to proteins A or G, or resin that has affinity and specificity for mouse IgG for the mouse antibody purification kits. After incubation of the antibody with the beads or resin, a simple wash procedure removes any unwanted substances. The purified antibody is then eluted and neutralized. This method cannot be used with samples containing dilute antibody in large volumes, eg, tissue culture supernatant.  

            Purification of antibodies from TCS

            TCS contains bovine IgG molecules from fetal bovine serum (FBS), which supplements cell growth media. These molecules need to be removed before carrying out conjugation reactions because they can compete with the antibody for labeling.

            Table 2. 

            Product nameUseConjugation kit compatibility
            TCS Antibody Purification Kit (ab109207)Suitable for purifying IgG from most species, not suitable for purifying goat IgG

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            Mouse TCS Antibody Purification Kit (ab128749)Suitable for purifying mouse IgG only 

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            Rat TCS Antibody Purification Kit (ab270562)Suitable for purifying rat IgG only

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            TCS Antibody Purification Kit – Nanoparticles (ab270554)Suitable for purifying IgG fractionsNanoparticle conjugation kits

            The purification kits listed in Table 2 use protein A or resins with a high affinity and specificity for mouse or rat IgG molecules coupled to agarose beads. The resin can selectively capture the antibody without co-purifying bovine IgG as its binding strength for bovine IgG molecules is minimal.

            Following incubation, the resin is loaded into a column, and a simple wash procedure removes unwanted substances. The purified antibody is then eluted and neutralized.

            Purification of antibodies from serum or ascites fluid

            Our antibody serum purification kits (Table 3) are prepared by coupling highly purified protein A or G to agarose beads to purify numerous components from antibodies generated from ascites fluid or serum.

            Table 3. 

            Product nameUseConjugation kit compatibility 
            Serum Antibody Purification Kit (Protein A) (ab109209)

            Suitable for purifying IgG fractions from serum and ascites fluid

            Not suitable for purifying goat IgG*

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            Serum Antibody Purification Kit (Protein G) (ab128751)Suitable for purifying IgG fractions from serum and ascites fluid

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            Antibody Purification Kit - Nanoparticles (ab204909)Purifies antibodies from crude samples such as ascites fluid or immune serumNanoparticle conjugation kits

            * Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies.

            The antibody is captured on the agarose beads and incubated with the resin. Following the incubation period, the resin is loaded into a column, and a simple wash procedure removes unwanted substances. The purified antibody is then eluted and neutralized.

            Magnetic antibody purification system

            The magnetic antibody purification system is a protein A-based antibody purification system with a unique set of elution and neutralization buffers used for the concentration of antibodies. It is also able to purify antibodies at a small scale with high recoveries.

            The antibody binds to protein A magnetic beads, which are subsequently captured with a magnetic stand to purify the antibody.

            Table 4.

            Product nameUseConjugation kit compatibility
            Magnetic Antibody Purification System (ab270532)

            Antibody concentration

            Suitable for purifying very low amounts of antibody 
            Compatible with all conjugation kits 

            Antibody concentration and clean-up kits

            Our antibody concentration and clean-up kits (Table 5) enable the quick and easy concentration of antibodies and proteins using a spin column.

            Table 5.

            Product nameUse**Conjugation kit compatibility
            Antibody Concentration And Clean-Up Kit (ab102778)

            Suitable for the concentration of antibodies and proteins

            Remove small additives such as azide, glycine, and Tris

            Lightning-Link® conjugation kits

            Oligonucleotide conjugation kits
            Antibody Concentration And Clean-Up Kit – Nanoparticles (ab204911)Suitable for the concentration of antibodies and proteins

            Remove small additives such as salt, citrate, and azide

            Gold and Magnetic Conjugation Kits
            Antibody Concentration and Clean Up Kit for Latex & Europium (ab269965)Suitable for the concentration of antibodies and proteins

            Remove small additives such as salt, citrate, and azide
            Latex and Europium conjugation kits

            ** It is essential to note that any buffer components with a molecular weight greater than 10 kDa, such as BSA, will also become concentrated during this process. If the additive concentration is incompatible for conjugation following concentration and resuspension, the appropriate purification kit should be used.

            These kits can also be used to remove any undesirable low molecular weight additives such as Tris, glycine, and sodium azide from the antibody storage buffer that passes through a filter, trapping the antibody, which is then recovered in a conjugation-friendly buffer. Removing sodium azide is particularly important if conjugating your antibody Horseradish Peroxidase (HRP) because sodium azide rapidly reduces HRP’s enzyme activity.


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