Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 抗体 [EPR22492-64] (ab252938)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22492-64] to KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] -
製品の詳細
Rabbit monoclonal [EPR22492-64] to KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged human KIR2DL1 (aa1-245), His and LIF-tagged human KIR2DL2 (aa26-221), His-tagged human KIR2DL3 (aa1-245), His-tagged human KIR2DS1 (aa22-221) and His-tagged human KIR2DS2 recombinant proteins (aa22-221). Flow Cyt: Human PBMC. IHC: human endometrium, colon cancer and gastric cancer
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22492-64 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab252938の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 39-60 kDa (predicted molecular weight: 34, 38, 39 kDa).
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Flow Cyt |
1/50.
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特記事項 |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 39-60 kDa (predicted molecular weight: 34, 38, 39 kDa). |
Flow Cyt
1/50. |
ターゲット情報
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細胞内局在
KIR2DL3: Cell membrane. KIR2DL1: Cell membrane. KIR2DL2: Cell membrane. KIR2DS1: Cell membrane. -
参照データベース
- Entrez Gene: 100132285 Human
- Entrez Gene: 3802 Human
- Entrez Gene: 3803 Human
- Entrez Gene: 3804 Human
- Entrez Gene: 3806 Human
- Omim: 604936 Human
- Omim: 604937 Human
- Omim: 604938 Human
see all
画像
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All lanes : Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] (ab252938) at 1/1000 dilution
Lane 1 : His-tagged human KIR2DL1 recombinant protein (aa1-245) 20 ng
Lane 2 : His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221) 20 ng
Lane 3 : His-tagged human KIR2DL3 recombinant protein (aa1-245) 20 ng
Lane 4 : His-tagged human KIR2DS1 recombinant protein (aa22-221) 20 ng
Lane 5 : His-tagged human KIR2DS2 recombinant protein (aa22-221) 20 ng
Lane 6 : His-tagged human KIR2DS3 recombinant protein (aa22-221) 20 ng
Lane 7 : His-tagged human KIR2DS4 recombinant protein (aa22-221) 20 ng
Lane 8 : His-tagged human KIR2DS5 recombinant protein (aa22-221) 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34, 38, 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 15 secs; Lanes 4-8: 3 mins.
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Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980) is observed.
Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) labeling KIR2DL1 with ab252938 at 1/50 (right) compared with a Rabbit monoclonal IgG (ab172730) (left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab252938 (Right). Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human colon cancer (PMID: 7749980) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human gastric cancer (PMID: 7749980) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab252938 は論文での使用が確認できていません。