Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain.
There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
Belongs to the kinesin-like protein family. Kinesin subfamily. Contains 1 kinesin-motor domain.
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Shows a Western blot of kinesin 5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-Kinesin 5C antibody (ab5630)Image from Dunn S et al, J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95. Epub 2008 Mar 11, Fig 2.
ab5630 staining Kinesin 5C in COS-7 cells by Immunocytochemistry/ Immunofluorescence. GFP-Kif5c behaviour in living cells. Cropped still images from a time-lapse recording of GFP-Kif5c puncta (green) moving along a MT labelled with mCherry–-tubulin (red) in a live COS-7 cell. The GFP-Kif5c puncta move from right to left. Scale bar, 2 µm. The graph shows the respective velocity of the GFP-Kif5c puncta seen over time, demonstrating the rapid changes in velocity of those puncta.
IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Leiva-Rodríguez T et al. ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons. Cell Death Dis9:626 (2018).
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Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep20:2792-2799 (2017).
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