Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (forebrain)
Immuno-precipitation step
Protein A
Specification
forebrain
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: 10mM HBS, 0.5% (v/v) Triton X-100
Dilution
1/4000
Additional data
Additional Notes
Lysis buffer was 10mM HEPES, pH, 7.4, containing 145mM NaCl, 1mM EGTA, 0.1mM MgCl2, 1% (v/v) Triton X-100 [(10 micrograms/ml) benzamidine, soybean trypsin inhibitor, chicken egg trypsin inhibitor, bacitracin and phenylmethyl sulfonic acid. Proteins were transferred to nitrocellulose at 14V for 16h at rt.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Kieran Brickley
Verified customer
投稿 May 14 2009