Anti-KIF5B 抗体 (ab5629)
Key features and details
- Rabbit polyclonal to KIF5B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF5B antibody
KIF5B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF5B -
由来種
Rabbit -
特異性
This antibody is specific for KIF5B and does not detect other kinesin isotypes. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab5629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/250. | |
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human KIF5B peptide (ab41782). |
ターゲット情報
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機能
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. -
配列類似性
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
Contains 1 kinesin motor domain. -
ドメイン
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
細胞内局在
Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons. - Information by UniProt
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参照データベース
- Entrez Gene: 3799 Human
- Entrez Gene: 16573 Mouse
- Omim: 602809 Human
- SwissProt: P33176 Human
- SwissProt: Q61768 Mouse
- Unigene: 327736 Human
- Unigene: 223744 Mouse
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別名
- Conventional kinesin heavy chain antibody
- KIF 5B antibody
- KIF5B antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.
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Western blot - Anti-KIF5B antibody (ab5629)Shows ab5629 used for a Western blot of Mouse neuronal cell extract.
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Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)ab5629 at 1/200 dilution staining the mouse oligodendrocyte cell line, Olineu, by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated secondary antibody.
This image is courtesy of an Abreview submitted by Katarina Trajko on 22 February 2006.
プロトコール
データシートおよび資料
参考文献 (10)
ab5629 は 10 報の論文で使用されています。
- Tigchelaar W et al. Hypertrophy induced KIF5B controls mitochondrial localization and function in neonatal rat cardiomyocytes. J Mol Cell Cardiol 97:70-81 (2016). PubMed: 27094714
- Du W et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell 37:326-336 (2016). PubMed: 27219061
- Carpentier DC et al. Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex. PLoS Pathog 11:e1004723 (2015). WB . PubMed: 25760349
- Brown AK et al. Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport. Biol Open 3:307-13 (2014). PubMed: 24705013
- Barry J et al. Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels. J Cell Sci 126:2027-41 (2013). PubMed: 23487040
- Hunt SD et al. Microtubule motors mediate endosomal sorting by maintaining functional domain organization. J Cell Sci 126:2493-501 (2013). PubMed: 23549789
- Loschi M et al. Dynein and kinesin regulate stress-granule and P-body dynamics. J Cell Sci 122:3973-82 (2009). ICC/IF . PubMed: 19825938
- Cardoso CM et al. Depletion of kinesin 5B affects lysosomal distribution and stability and induces peri-nuclear accumulation of autophagosomes in cancer cells. PLoS One 4:e4424 (2009). WB, ICC/IF ; Human . PubMed: 19242560
- DeBoer SR et al. Conventional kinesin holoenzymes are composed of heavy and light chain homodimers. Biochemistry 47:4535-43 (2008). PubMed: 18361505
- Brickley K et al. GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin. J Biol Chem 280:14723-32 (2005). PubMed: 15644324
