Anti-KIF5B 抗体 (ab5629)
Key features and details
- Rabbit polyclonal to KIF5B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-KIF5B antibody
KIF5B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF5B -
由来種
Rabbit -
特異性
This antibody is specific for KIF5B and does not detect other kinesin isotypes. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5629の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
1/250.
|
| WB |
1/2000 - 1/6000. Predicted molecular weight: 110 kDa.Can be blocked with Human KIF5B peptide (ab41782).
|
| 特記事項 |
|---|
|
ICC/IF
1/250. |
|
WB
1/2000 - 1/6000. Predicted molecular weight: 110 kDa.Can be blocked with Human KIF5B peptide (ab41782). |
ターゲット情報
-
機能
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. -
配列類似性
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
Contains 1 kinesin motor domain. -
ドメイン
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
細胞内局在
Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons. - Information by UniProt
-
参照データベース
- Entrez Gene: 3799 Human
- Entrez Gene: 16573 Mouse
- Entrez Gene: 117550 Rat
- Omim: 602809 Human
- SwissProt: P33176 Human
- SwissProt: Q61768 Mouse
- SwissProt: Q2PQA9 Rat
- Unigene: 327736 Human
see all -
別名
- Conventional kinesin heavy chain antibody
- KIF 5B antibody
- KIF5B antibody
see all
画像
-
Western blot - Anti-KIF5B antibody (ab5629)All lanes : Anti-KIF5B antibody (ab5629) at 1/2000 dilution
Lane 1 : Mouse brain Whole cell extract
Lane 2 : Mouse lung Whole cell extract
Lane 3 : Rat brain Whole cell extract
Lane 4 : Rat liver Whole cell extract
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L),HRP at 1/4000 dilution
Predicted band size: 110 kDa -
Western blot - Anti-KIF5B antibody (ab5629)All lanes : Anti-KIF5B antibody (ab5629) at 1/4000 dilution
Lane 1 : Mouse Cerebellum whole cell extract
Lane 2 : Rat Brain whole cell extract
Lane 3 : Mouse Brain whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 110 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (10)
ab5629 は 10 報の論文で使用されています。
- Tigchelaar W et al. Hypertrophy induced KIF5B controls mitochondrial localization and function in neonatal rat cardiomyocytes. J Mol Cell Cardiol 97:70-81 (2016). PubMed: 27094714
- Du W et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell 37:326-336 (2016). PubMed: 27219061
- Carpentier DC et al. Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex. PLoS Pathog 11:e1004723 (2015). WB . PubMed: 25760349
- Brown AK et al. Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport. Biol Open 3:307-13 (2014). PubMed: 24705013
- Barry J et al. Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels. J Cell Sci 126:2027-41 (2013). PubMed: 23487040
- Hunt SD et al. Microtubule motors mediate endosomal sorting by maintaining functional domain organization. J Cell Sci 126:2493-501 (2013). PubMed: 23549789
- Loschi M et al. Dynein and kinesin regulate stress-granule and P-body dynamics. J Cell Sci 122:3973-82 (2009). ICC/IF . PubMed: 19825938
- Cardoso CM et al. Depletion of kinesin 5B affects lysosomal distribution and stability and induces peri-nuclear accumulation of autophagosomes in cancer cells. PLoS One 4:e4424 (2009). WB, ICC/IF ; Human . PubMed: 19242560
- DeBoer SR et al. Conventional kinesin holoenzymes are composed of heavy and light chain homodimers. Biochemistry 47:4535-43 (2008). PubMed: 18361505
- Brickley K et al. GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin. J Biol Chem 280:14723-32 (2005). PubMed: 15644324
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com