Anti-KIF4A/KIF4 抗体 (ab122227)
Key features and details
- Rabbit polyclonal to KIF4A/KIF4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KIF4A/KIF4 antibody
KIF4A/KIF4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF4A/KIF4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human KIF4A/KIF4 aa 994-1075.
Sequence:LTLLQVASRQKHLPKDTLLSPDSSFEYVPPKPKPSRVKEKFLEQSMDIED LKYCSEHSVNEHEDGDGDDDEGDDEEWKPTKL
Database link: O95239 -
ポジティブ・コントロール
- WB: RT-4 and U-251 MG cell lysates IHC-P: Human bone marrow tissue ICC/IF: Human cell line U-2 OS
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122227の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
組織特異性
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
配列類似性
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
細胞内局在
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
-
参照データベース
- Entrez Gene: 24137 Human
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- Unigene: 648326 Human
-
別名
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
画像
-
All lanes : Anti-KIF4A/KIF4 antibody (ab122227) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate -
ab122227, at a 1/300 dilution, staining KIF4A/KIF4 in paraffin embedded Human bone marrow tissue by Immunohistochemistry.
-
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli using ab122227.
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100 -
Immunohistochemical staining of Human bone marrow shows strong nuclear positivity in hematopoietic cells using ab122227
-
ab122227, at 4 µg/ml, staining KIF4A/KIF4 in Human cell line U-2 OS by Immunofluorescence. Antibody staining is shown in green.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (10)
ab122227 は 10 報の論文で使用されています。
- Zheng P et al. KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3. Int J Mol Med 47:N/A (2021). PubMed: 33846765
- Pan J et al. Identification of KIF4A as a pan-cancer diagnostic and prognostic biomarker via bioinformatics analysis and validation in osteosarcoma cell lines. PeerJ 9:e11455 (2021). PubMed: 34055488
- Wang L et al. Identification of KIF4A as a prognostic biomarker for esophageal squamous cell carcinoma. Aging (Albany NY) 13:24050-24070 (2021). PubMed: 34775374
- Chen J et al. KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion. Biomed Res Int 2021:8249293 (2021). PubMed: 34805404
- Feng S et al. miR-29c-3p regulates proliferation and migration in ovarian cancer by targeting KIF4A. World J Surg Oncol 18:315 (2020). PubMed: 33261630
- Liu G et al. The kinesin motor protein KIF4A as a potential therapeutic target in renal cell carcinoma. Invest New Drugs 38:1730-1742 (2020). PubMed: 32533288
- Chen J et al. KIF4A: A potential biomarker for prediction and prognostic of prostate cancer. Clin Invest Med 43:E49-59 (2020). PubMed: 32971585
- Jungwirth G et al. Identification of KIF11 As a Novel Target in Meningioma. Cancers (Basel) 11:N/A (2019). PubMed: 30991738
- Matsumoto Y et al. Enhanced expression of KIF4A in colorectal cancer is associated with lymph node metastasis. Oncol Lett 15:2188-2194 (2018). PubMed: 29434924
- Hou PF et al. KIF4A facilitates cell proliferation via induction of p21-mediated cell cycle progression and promotes metastasis in colorectal cancer. Cell Death Dis 9:477 (2018). PubMed: 29706624