Anti-KIF1A 抗体 [EPR11790(B)] (ab180153)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11790(B)] to KIF1A
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-KIF1A antibody [EPR11790(B)]
KIF1A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11790(B)] to KIF1A -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
適用なし: IHC-P -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide within Human KIF1A aa 900-1000. The exact sequence is proprietary.
Database link: Q12756 -
ポジティブ・コントロール
- HEK-293 cells, Human fetal brain lysates, SH-SY5Y cells, U-87 MG cells, rat hypothalamus lysate and rat brain lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11790(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180153の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/5000 - 1/10000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).
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ICC/IF |
1/100.
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IP |
1/50.
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特記事項 |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/5000 - 1/10000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa). |
ICC/IF
1/100. |
IP
1/50. |
ターゲット情報
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機能
Motor for anterograde axonal transport of synaptic vesicle precursors. -
配列類似性
Belongs to the kinesin-like protein family. Unc-104 subfamily.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Contains 1 PH domain. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 547 Human
- Entrez Gene: 363288 Rat
- Omim: 601255 Human
- SwissProt: Q12756 Human
- Unigene: 516802 Human
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別名
- ATSV antibody
- Axonal transporter of synaptic vesicles antibody
- C2orf20 antibody
see all
画像
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All lanes : Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/1000 dilution (purified)
Lane 1 : Rat hypothalamus lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 191 kDa
Observed band size: 191 kDaBlocking and diluting buffer and concentration: 5% NFDM /TBST.
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Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling KIF1A with purified ab180153 at 1/100 dilution (9.2μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. Ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the secondary antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as the nuclear counter stain.
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ab180153 (purified) at 1/50 dilution (2ug) immunoprecipitating KIF1A in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug
Lane 2 (+): ab180153 + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180153 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Intracellular Flow Cytometry analysis ofSH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling KIF1A with purifiedab180153 at 1/90 dilution (10 ug/ml).Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution.Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabelled control, cells without incubation with primary antibody and secondary antibody.
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Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (purified) + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 191 kDa
Observed band size: 191 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling KIF1A with unpurified ab180153 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
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Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/20000 dilution (unpurified) + 293 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 191 kDa -
Western blot analysis of 293 cell lysate immunoprecipitated using unpurified ab180153 at 1/30 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedSH-SY5Y cells labeling KIF1A with unpurified ab180153 at 1/150 dilution (red), compared to a Rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
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Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (unpurified) + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 191 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab180153 は 6 報の論文で使用されています。
- Aguilera C et al. The Novel KIF1A Missense Variant (R169T) Strongly Reduces Microtubule Stimulated ATPase Activity and Is Associated With NESCAV Syndrome. Front Neurosci 15:618098 (2021). PubMed: 34121983
- Mou Y et al. MFN2 Deficiency Impairs Mitochondrial Transport and Downregulates Motor Protein Expression in Human Spinal Motor Neurons. Front Mol Neurosci 14:727552 (2021). PubMed: 34602978
- Diwaker D et al. Deletion of the Pseudorabies Virus gE/gI-US9p complex disrupts kinesin KIF1A and KIF5C recruitment during egress, and alters the properties of microtubule-dependent transport in vitro. PLoS Pathog 16:e1008597 (2020). PubMed: 32511265
- Pesaresi M et al. Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features. Biol Sex Differ 9:6 (2018). WB ; Rat . PubMed: 29351809
- Wang J et al. Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats. Front Cell Neurosci 11:432 (2017). PubMed: 29375318
- Du W et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell 37:326-336 (2016). PubMed: 27219061