Anti-KIF14 抗体 (ab3747)
Key features and details
- Rabbit polyclonal to KIF14
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KIF14 antibody
KIF14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF14 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human KIF14 aa 900-1000.
Database link: Q15058 -
ポジティブ・コントロール
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3747の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
|
|
IP |
Use at 1-4 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 190 kDa. |
IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
-
機能
Plays an essential role in cytokinesis. -
配列類似性
Belongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain. -
発生段階
Up-regulated in cells progressing through G2/M phase. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring. - Information by UniProt
-
参照データベース
- Entrez Gene: 9928 Human
- Omim: 611279 Human
- SwissProt: Q15058 Human
- Unigene: 3104 Human
-
別名
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
see all
画像
-
All lanes : Anti-KIF14 antibody (ab3747) at 1/2000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 190 kDa
Exposure time: 10 seconds -
Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Lane 1 - Input.
Lane 2 - IP.
Lane 3 - Control IgG IP.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab3747 は 1 報の論文で使用されています。
- Schiewek J et al. Clinical relevance of cytoskeleton associated proteins for ovarian cancer. J Cancer Res Clin Oncol 144:2195-2205 (2018). PubMed: 30094535