Anti-KIF14 抗体 (ab3746)
Key features and details
- Rabbit polyclonal to KIF14
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIF14 antibody
KIF14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIF14 -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human KIF14 aa 50-150.
Database link: Q15058 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 1-4 µg/mg of lysate.
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特記事項 |
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IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
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機能
Plays an essential role in cytokinesis. -
配列類似性
Belongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain. -
発生段階
Up-regulated in cells progressing through G2/M phase. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring. - Information by UniProt
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参照データベース
- Entrez Gene: 9928 Human
- Omim: 611279 Human
- SwissProt: Q15058 Human
- Unigene: 3104 Human
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別名
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
see all
画像
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KIF14 was immunoprecipitated from 50 µg/ml and 1 mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab3746 at 3 µg/ml. Western blot was performed from the immunoprecipitate using ab3746 at 1 µg/ml.
Lane 1: HeLa whole cell lysate 50 µg (Input).
Lane 2: ab3746 IP in HeLa whole cell lysate.
Lane 3: Ctrl IgG instead of ab3746.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab3746 は 7 報の論文で使用されています。
- Vukušic K et al. Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Dev Cell 56:1253-1267.e10 (2021). PubMed: 33910056
- Malaby HLH et al. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol 218:1218-1234 (2019). PubMed: 30709852
- Reilly ML et al. Loss-of-function mutations in KIF14 cause severe microcephaly and kidney development defects in humans and zebrafish. Hum Mol Genet 28:778-795 (2019). PubMed: 30388224
- Wang ZZ et al. KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer. Int J Oncol 53:1939-1952 (2018). PubMed: 30226594
- Moawia A et al. Mutations of KIF14 cause primary microcephaly by impairing cytokinesis. Ann Neurol 82:562-577 (2017). PubMed: 28892560
- Sgrò F et al. Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation. Cell Death Differ 23:801-13 (2016). PubMed: 26586574
- Carleton M et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF ; Human . PubMed: 16648480