Anti-KIF14 抗体 (ab3746)

Submit a review Q&A (3)References (6)
Immunoprecipitation - Anti-KIF14 antibody (ab3746)

    Key features and details

    • Rabbit polyclonal to KIF14
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-KIF14 antibody
      KIF14 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to KIF14

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: IPmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla

    • 免疫原

      Synthetic peptide corresponding to Human KIF14 aa 50-150.
      Database link: Q15058

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3746の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IP
    Use at 1-4 µg/mg of lysate.
    特記事項
    IP
    Use at 1-4 µg/mg of lysate.

    ターゲット情報

    • 機能

      Plays an essential role in cytokinesis.

    • 配列類似性

      Belongs to the kinesin-like protein family.
      Contains 1 FHA domain.
      Contains 1 kinesin-motor domain.

    • 発生段階

      Up-regulated in cells progressing through G2/M phase.

    • 細胞内局在

      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
    • Information by UniProt

    • 参照データベース

      • 別名

        • KIAA0042 antibody
        • KIF 14 antibody
        • KIF14 antibody
        • KIF14_HUMAN antibody
        • Kinesin Family Member 14 antibody
        • Kinesin like protein KIF14 antibody
        • Kinesin-like protein KIF14 antibody
        • MGC142302 antibody
        see all

      画像

      • Immunoprecipitation - Anti-KIF14 antibody (ab3746)
        Immunoprecipitation - Anti-KIF14 antibody (ab3746)

        KIF14 was immunoprecipitated from 50 µg/ml and 1 mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab3746 at 3 µg/ml. Western blot was performed from the immunoprecipitate using ab3746 at 1 µg/ml.

        Lane 1: HeLa whole cell lysate 50 µg (Input). 

        Lane 2: ab3746 IP in HeLa whole cell lysate. 

        Lane 3: Ctrl IgG instead of ab3746.

      参考文献 (6)

      ab3746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3746 は 6 報の論文で使用されています。

      • Vukušic K  et al. Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Dev Cell 56:1253-1267.e10 (2021). PubMed: 33910056
      • Malaby HLH  et al. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol 218:1218-1234 (2019). PubMed: 30709852
      • Reilly ML  et al. Loss-of-function mutations in KIF14 cause severe microcephaly and kidney development defects in humans and zebrafish. Hum Mol Genet 28:778-795 (2019). PubMed: 30388224
      • Wang ZZ  et al. KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer. Int J Oncol 53:1939-1952 (2018). PubMed: 30226594
      • Sgrò F  et al. Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation. Cell Death Differ 23:801-13 (2016). PubMed: 26586574
      • Carleton M  et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF ; Human . PubMed: 16648480

      レビューと Q&A

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      1-3 of 3 Abreviews or Q&A

      Question

      Thank you for your prompt response.
      Would it be possible to have
      1. anti-periplakin, ab131269 as a direct replacement (we are willing to wait 1-2 weeks for this antibody)
      However, has there is a delay on the KIF14 antibody would it be possible to replace this antibody with Anti-Vitronectin antibody [VN58-1] (ab13413)
      as this is available immediately

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedtwo free of charge replacements with the order numbers ***** for the anti-Vitronectin ab13413 and **** for the anti-Periplakin antibodyab131269.

      To check the status of the orders please contact our Customer Service team and reference these numbers.

      Please note that these free of charge replacements vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      Our lab recent purchased a large batch of antibodies from your company. Purchase Order *****. Two of which are not working correctly.
      I have attachted a word document containing Western blots using the two primary antibodies:-
      Anti-Periplakin antibody - (ab72422)
      Anti-KIF14 antibody - (ab3746)
      These western blots have been exposed for 15 minutes. Whereas, the Abcam info sheets state 10 seconds required.
      The Periplakin blot shows a band at approximately 55 kDa that appeared after 10 seconds, however the band just below 200 kDa (which I believe to be periplakin) wasn't picked up until 15 minutes exposure.
      The KIF14 blots shows a band at just below 200kDa (which I believe to be KIF14), however this wasn't picked up until 15 minutues exposure.
      The samples that I used to perform this experiment, are the same samples used to conduct MS/MS-SILAC and the results of this confirmed that there was a drastic increase in these proteins in these samples compared to mock whole cell lysate. Hence I should be seeing a strong band for both KIF14 and Periplakin.
      Is there any alternativies or a differnt batch of these antibodies that you can send us?
      I look forward to hearing from you.

      Read More
      Answer

      Thank you for contacting us and for providing this further information.

      As explained over the phone,I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement, a refund or credit note in compensation.
      As replacement please find below the list of alternative productswe can send to you. Please note that these 3 antibodies are currently out of stock and should be delivered to you withina week.

      anti-KIF14, ab3747, https://www.abcam.com/ab3747
      anti-KIF14, ab71155, https://www.abcam.com/ab71155
      anti-periplatin, ab131269, https://www.abcam.com/ab131269




      I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Question

      Dear Sir/Madam,
      Our lab (Adrian Whithouse lab, Leeds University) recent purchased a large batch of antibodies from your company. Purchase Order 4500686042. Two of which are not working correctly.
      I have attachted a word document containing Western blots using the two primary antibodies:-
      Anti-Periplakin antibody - (ab72422)
      Anti-KIF14 antibody - (ab3746)
      These western blots have been exposed for 15 minutes. Whereas, the Abcam info sheets state 10 seconds required.
      The Periplakin blot shows a band at approximately 55 kDa that appeared after 10 seconds, however the band just below 200 kDa (which I believe to be periplakin) wasn't picked up until 15 minutes exposure.
      The KIF14 blots shows a band at just below 200kDa (which I believe to be KIF14), however this wasn't picked up until 15 minutues exposure.
      The samples that I used to perform this experiment, are the same samples used to conduct MS/MS-SILAC and the results of this confirmed that there was a drastic increase in these proteins in these samples compared to mock whole cell lysate. Hence I should be seeing a strong band for both KIF14 and Periplakin.
      Is there any alternativies or a differnt batch of these antibodies that you can send us?
      I look forward to hearing from you.
      Kind regards

      Read More
      Answer

      Thank you for contacting us and for sending an image of your blot.

      I am sorry to hear you are experiencing difficulties with two of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      I am addingsome questions belowso that we can gather further information regarding the samples tested and the protocol used. Once we receive the answers, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

      Samples
      Species of the sample :
      Type of the tissue/cell lysate :

      Transfer
      Transfer conditions (time and temperature, wet or semi-dry):
      Transfer buffer :
      Protein transfer verified?

      Blocking
      Blocking agent and concentration :

      Primary antibody
      concentration or dilution :
      Diluent buffer :
      Incubation time :
      Incubation temperature :

      I look forward to receiving your reply.

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