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Anti-Kidins220 抗体 (ab97345)

Submit a review Q&A (1)References (1)
Western blot - Anti-Kidins220 antibody (ab97345)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kidins220 antibody (ab97345)

Key features and details

  • Rabbit polyclonal to Kidins220
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Kidins220 antibody
    Kidins220 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Kidins220

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1706-1771 of Human Kidins220 (NP_065789).

  • ポジティブ･コントロール

    • 293T, H1299, HeLaS3, HepG2, MOLT4, Raji cell lysates and SW480 xenograft.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab97345の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 197 kDa.
IHC-P
1/100 - 1/500.
特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 197 kDa.
IHC-P
1/100 - 1/500.

ターゲット情報

  • 機能

    Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.

  • 組織特異性

    Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.

  • 配列類似性

    Contains 12 ANK repeats.
    Contains 1 KAP NTPase domain.

  • ドメイン

    The transmembrane domain mediates interaction with NTRK1.

  • 翻訳後修飾

    Tyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF).

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Ankyrin repeat-rich membrane-spanning protein antibody
      • arms antibody
      • KDIS_HUMAN antibody
      • kidins220 antibody
      • Kinase D interacting substance of 220 kDa antibody
      • Kinase D-interacting substrate of 220 kDa antibody
      • rgd 619949 antibody
      see all

    画像

    • Western blot - Anti-Kidins220 antibody (ab97345)
      Western blot - Anti-Kidins220 antibody (ab97345)
      All lanes : Anti-Kidins220 antibody (ab97345) at 1/1000 dilution

      Lane 1 : H1299 whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 197 kDa



      5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kidins220 antibody (ab97345)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kidins220 antibody (ab97345)
      ab97345 at 1/500 dilution staining Kidins220 in paraffin-embedded SW480 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    参考文献 (1)

    ab97345 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab97345 は 1 報の論文で使用されています。

    • Wang Y  et al. MiR-4638-5p inhibits castration resistance of prostate cancer through repressing Kidins220 expression and PI3K/AKT pathway activity. Oncotarget 7:47444-47464 (2016). PubMed: 27329728

    レビューと Q&A

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    Question

    Tengo una duda respecto a un control positivo para ab97345. ¿Podría ser carcinoma de colon? Dependiendo de los artículos, encuentro diferentes cosas y en la página de abcam pone líneas celulares, pero no tejidos. Disculpa las molestias y muchas gracias por tu ayuda.

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    Answer

    He buscado tejidos de elevada expresión de esta proteína, y efectivamente, el carcinoma colorrectal (o cáncer de colon) es uno de los que más tinción presenta al teñirlo con el anticuerpo correspondiente.   Otros tejidos con elevada expresión de KIDINS220 son: laringe, cerebro, glándula pituitaria, o tejidos tumorales como cabeza y cuello, esófago y células germinales. Te adjunto el link del perfil de expresión de la proteína, donde puedes encontrar su variación por tejidos: http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.9873 Las líneas celulares que se mencionan en la ficha técnica son sugerencias para Western Blot. Espero haberte ayudado. Por favor, no dudes en consultaros para cualquier otra duda o sugerencia.  

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