Anti-KIAA0802 抗体 (ab100914)
Key features and details
- Rabbit polyclonal to KIAA0802
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KIAA0802 antibody -
製品の詳細
Rabbit polyclonal to KIAA0802 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
A region between amino acids 926-976 of Human KIAA0802 (AAH40542.2).
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ポジティブ･コントロール
- HeLa lysate
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特記事項
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100914の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 209 kDa.
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| IP |
Use at 5-15 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 209 kDa. |
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IP
Use at 5-15 µg/mg of lysate. |
ターゲット情報
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機能
Microtubule-associated factor involved in the late phase of epithelial polarization and microtubule dynamics regulation. Plays a role in the development and maintenance of non-centrosomal microtubule bundles at the lateral membrane in polarized epithelial cells. -
配列類似性
Belongs to the SOGA family. -
細胞内局在
Lateral cell membrane. Apical cell membrane. Cytoplasm, cytoskeleton, spindle pole. Midbody. Cytoplasm, cytoskeleton. Colocalized with microtubules at the base of cilia. Gradually accumulates on the apicobasal microtubule bundles during epithelial cell polarization (By similarity). Colocalized with the apicobasal microtubule bundles running beneath the lateral membrane. Colocalized with microtubule bundles in the spindle pole in mitotic cells and in the midbodies at the end of cytokinesis. - Information by UniProt
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参照データベース
- Entrez Gene: 23255 Human
- SwissProt: Q9Y4B5 Human
- Unigene: 707920 Human
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別名
- Coiled-coil domain-containing protein 165 antibody
- Microtubule cross-linking factor 1 antibody
- MTCL1 antibody
see all
画像
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Western blot - Anti-KIAA0802 antibody (ab100914)All lanes : Anti-KIAA0802 antibody (ab100914) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 50 µg
Lane 2 : HeLa Whole Cell Lysate at 15 µg
Lane 3 : HeLa Whole Cell Lysate at 5 µg
Predicted band size: 209 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-KIAA0802 antibody (ab100914)ab100914 at 1 µg/ml staining KIAA0802 in HeLa cell lysate immunoprecipitated using ab100914 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 3 minutes
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab100914 は論文での使用が確認できていません。
