Anti-KIAA0652/ATG13 抗体 (ab105392)
Key features and details
- Rabbit polyclonal to KIAA0652/ATG13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-KIAA0652/ATG13 antibody
KIAA0652/ATG13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KIAA0652/ATG13 -
由来種
Rabbit -
特異性
ab105392 is predicted to not cross-react with other ATG members. Two isoforms of KIAA0652/ATG13 are known to exist; this antibody will recognize both isoforms.
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アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human KIAA0652/ATG13 (C terminal).
Database link: NP_001136145 -
ポジティブ･コントロール
- WB: Rat heart tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105392の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. -
配列類似性
Belongs to the ATG13 metazoan family. -
翻訳後修飾
Phosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. -
細胞内局在
Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. - Information by UniProt
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参照データベース
- Entrez Gene: 9776 Human
- Entrez Gene: 51897 Mouse
- Entrez Gene: 362164 Rat
- SwissProt: O75143 Human
- SwissProt: Q91YI1 Mouse
- Unigene: 127403 Human
- Unigene: 28492 Mouse
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別名
- ATG13 antibody
- ATG13 autophagy related 13 homolog (S. cerevisiae) antibody
- ATG13_HUMAN antibody
see all
画像
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Western blot - Anti-KIAA0652/ATG13 antibody (ab105392)All lanes : Anti-KIAA0652/ATG13 antibody (ab105392) at 1 µg/ml
Lane 1 : Rat heart tissue lysate without blocking peptide
Lane 2 : Rat heart tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 57 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab105392 は 6 報の論文で使用されています。
- Wang S et al. miR-654-5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Mol Med Rep 23:N/A (2021). PubMed: 33846806
- Stavoe AK et al. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. Elife 8:N/A (2019). PubMed: 31309927
- Zhou M et al. Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway. Front Pharmacol 10:603 (2019). PubMed: 31214032
- Shin JH et al. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy 15:1495-1505 (2019). PubMed: 30821607
- Liu W et al. Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway. Int J Mol Med 37:309-18 (2016). WB . PubMed: 26647915
- Huang J et al. HMGB1 promotes drug resistance in osteosarcoma. Cancer Res 72:230-8 (2012). WB ; Human . PubMed: 22102692
