Name: Anti-Ki67 antibody [SP6], prediluted
Brand: RabMAb
SKU: ab21700
Rating: 5 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP6] to Ki67, prediluted
Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)
Knockout validated
Reacts with: Mouse, Human

Alexa Fluor® 647 Carrier Free

製品名

Anti-Ki67 antibody [SP6], prediluted
Ki67 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [SP6] to Ki67, prediluted
由来種

Rabbit
特異性

ab21700 recognises Ki67.
アプリケーション

適用あり: WB, ICC, IHC-P, Flow Cyt (Intra)more details
種交差性

交差種: Mouse, Human
交差が予測される動物種: Rat
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Ramos and HeLa cell lysates. IHC-P: Human tonsil and thymus tissue; Human endometrial and breast adenocarcinoma tissue. ICC: HeLa and HAP1 cells. Flow Cyt (intra): HAP1 cells.
特記事項
This product was switched from hybridoma to recombinant format on 25th October 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA

Inert stabilizer
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

SP6
アイソタイプ

IgG
研究分野
- Neuroscience
- Development

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human MKI67 (Ki67) knockout HeLa cell line (ab255407)
KO cell lysates
- Human MKI67 (Ki67) knockout HeLa cell lysate (ab263762)
KO cell pellets
- Human MKI67 (Ki67) knockout HeLa cell pellet (ab278924)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab21700の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		Use at an assay dependent concentration. Predicted molecular weight: 358 kDa.
ICC		Use at an assay dependent concentration. Use at an assay dependent concentration. If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min
IHC-P	(2)	1/1. Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
Flow Cyt (Intra)		Use at an assay dependent concentration.

特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 358 kDa.
ICC Use at an assay dependent concentration. Use at an assay dependent concentration. If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min
IHC-P 1/1. Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
Flow Cyt (Intra) Use at an assay dependent concentration.

機能

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
配列類似性

Contains 1 FHA domain.
Contains 16 K167R repeats.
Contains 1 PP1-binding domain.
発生段階

Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
翻訳後修飾

Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
細胞内局在

Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
Target information above from: UniProt accession P46013 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4288 Human
- Entrez Gene: 17345 Mouse
- Entrez Gene: 246042 Rat
- Omim: 176741 Human
- SwissProt: P46013 Human
- SwissProt: E9PVX6 Mouse
- SwissProt: Q61769 Mouse
- Unigene: 689823 Human
- Unigene: 80976 Human
- Unigene: 4078 Mouse
see all
別名
- Antigen identified by monoclonal antibody Ki 67 antibody
- Antigen identified by monoclonal antibody Ki-67 antibody
- Antigen KI-67 antibody
- Antigen KI67 antibody
- Antigen Ki67 antibody
- KI67_HUMAN antibody
- KIA antibody
- Marker of proliferation Ki-67 antibody
- MIB 1 antibody
- MIB antibody
- MKI67 antibody
- PPP1R105 antibody
- Proliferation marker protein Ki-67 antibody
- Proliferation related Ki 67 antigen antibody
- Protein phosphatase 1 regulatory subunit 105 antibody
- RP11-380J17.2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Ki67 with ab21700.
Western blot - Anti-Ki67 antibody [SP6], prediluted (ab21700)

All lanes : Anti-Ki67 antibody [SP6] (ab16667) at 1/100 dilution

Lane 1 : Ramos cell lysate
Lane 2 : Wild-type HeLa cell lysate
Lane 3 : MKI67 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 358 kDa
Observed band size: 359 kDa why is the actual band size different from the predicted?

This data was developed using the same antibody clone in a different buffer formulation (ab16667).

Lanes 1 - 3: Merged signal (red and green). Green - ab16667 observed at 359 kDa. Red - loading control, ab130007 observed at 125 kDa.

ab16667 was shown to react with Ki67 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255407 (knockout cell lysate ab263762) was used. Wild-type and Ki67 knockout samples were subjected to SDS-PAGE. ab16667 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 100 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry - Anti-Ki67 antibody [SP6], prediluted (ab21700)

This data was developed using ab16667, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, None permeabilized parental HAP1 cells labelling Ki67 with ab16667 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nucleolar staining in parental HAP1cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).
Immunocytochemistry - Anti-Ki67 antibody [SP6], prediluted (ab21700)

This data was developed using ab16667, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, None permeabilized HeLa cells labelling Ki67 with ab16667 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nucleolar staining in HeLa cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).
Flow Cytometry (Intracellular) - Anti-Ki67 antibody [SP6], prediluted (ab21700)

This data was developed using ab16667, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) cells labelling Ki67 with ab16667 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial adenocarcinoma tissue, staining Ki67 with ab21700.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, staining Ki67 with ab21700.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (ab21700)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast adenocarcinoma tissue, staining Ki67 with ab21700.
Anti-Ki67 antibody [SP6], prediluted (ab21700)

Immunohistochemistry protocols

Click here to view the general protocols

SDS download

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Datasheet download

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ab21700 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab21700 は 21 報の論文で使用されています。

Zhang H et al. MicroRNA-545 suppresses progression of ovarian cancer through mediating PLK1 expression by a direct binding and an indirect regulation involving KDM4B-mediated demethylation. BMC Cancer 21:163 (2021). PubMed: 33588776
Zhang YL et al. Progesterone suppresses the progression of colonic carcinoma by increasing the activity of the GADD45a/JNK/c-Jun signalling pathway. Oncol Rep 45:N/A (2021). PubMed: 33846816
Wang H et al. lncRNA ZFAS1 promotes the ox-LDL induced proliferation, invasion and migration of vascular smooth muscle cells. Exp Ther Med 22:835 (2021). PubMed: 34149881
Zhang S et al. Hsa_circ_0007494 suppresses prostate cancer progression via miR-616/PTEN axis. Exp Cell Res 395:112233 (2020). PubMed: 32810511
Lou Q et al. miR-448 targets IDO1 and regulates CD8+ T cell response in human colon cancer. J Immunother Cancer 7:210 (2019). PubMed: 31391111

View all Publications for this product

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH6

Sample

Human Tissue sections (Placenta)

Specification

Placenta

Permeabilization

Yes - Triton 1%

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Nov 21 2014

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Sample

Human Cell (HEK 293T)

Specification

HEK 293T

Permeabilization

Yes - Triton 1%

Fixative

Formaldehyde

Abcam user community

Verified customer

投稿 Nov 18 2014

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Pancreas)

Specification

Pancreas

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6

Permeabilization

Blocking step

FBS / BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

投稿 Feb 27 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

KO cell pellets

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

発生段階

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (21)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [SP6], prediluted

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ki67 antibody [SP6], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [SP6], prediluted