Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human colon carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
No
Specification
Human colon carcinoma
Blocking step
Background Punisher as blocking agent for 10 minute(s) · Concentration: 100%
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBST (0.1% Tween20)
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Signalstain® Boost IHC Detection Reagent (HRP, Rab
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Peroxide block was performed for 5mins after tissue deparaffinize in xylene.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 23 2021