Anti-Ki67 抗体 [MKI67/2465] (ab238020)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2465] (ab238020)](https://www.abcam.co.jp/ps/products/238/ab238020/Images/ab238020-343075-Ki67antibodyMKI672465-IHC-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2465] (ab238020)")
Key features and details
- Mouse monoclonal [MKI67/2465] to Ki67
- Suitable for: Protein Array, Flow Cyt, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-Ki67 antibody [MKI67/2465]
Ki67 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MKI67/2465] to Ki67 -
由来種
Mouse -
アプリケーション
適用あり: Protein Array, Flow Cyt, IHC-P, ICCmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human Ki67 aa 2293-2478. The exact sequence is proprietary.
Database link: P46013 -
ポジティブ･コントロール
- IHC-P: Human tonsil tissue. ICC: MCF7 cells. Flow: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A/G purified -
特記事項（精製）
Purified from Bioreactor concentrate. -
ポリ/モノ
モノクローナル -
クローン名
MKI67/2465 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab238020の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Protein Array |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
1-2 µg/million cells |
|
| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml.
Primary incubation for 30 minutes at room temperature. |
| ICC | (3) |
Use a concentration of 1 - 2 µg/ml.
|
| 特記事項 |
|---|
|
Protein Array
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. 1-2 µg/million cells |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Primary incubation for 30 minutes at room temperature. |
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ICC
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed. -
配列類似性
Contains 1 FHA domain.
Contains 16 K167R repeats.
Contains 1 PP1-binding domain. -
発生段階
Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815). -
翻訳後修飾
Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. -
細胞内局在
Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). - Information by UniProt
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参照データベース
- Entrez Gene: 4288 Human
- Omim: 176741 Human
- SwissProt: P46013 Human
- Unigene: 689823 Human
- Unigene: 80976 Human
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別名
- Antigen identified by monoclonal antibody Ki 67 antibody
- Antigen identified by monoclonal antibody Ki-67 antibody
- Antigen KI-67 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2465] (ab238020)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab238020 at 2 μg/ml in immunohistochemical analysis.
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![Flow Cytometry - Anti-Ki67 antibody [MKI67/2465] (ab238020)](https://www.abcam.co.jp/ps/products/238/ab238020/Images/ab238020-343077-ki67antibodyMKI672465-flow-cytometry-hela-human.jpg)
Flow Cytometry - Anti-Ki67 antibody [MKI67/2465] (ab238020)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ki67 with ab238020 at 2 μg per 106 cells (blue) compared to an isotype control (red). A Goat Anti-mouse CF488 secondary antibody was used.
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![Immunocytochemistry - Anti-Ki67 antibody [MKI67/2465] (ab238020)](https://www.abcam.co.jp/ps/products/238/ab238020/Images/ab238020-343076-ki67antibodyMKI672465-ICC-MCF7-human.jpg)
Immunocytochemistry - Anti-Ki67 antibody [MKI67/2465] (ab238020)MCF7 (human breast adenocarcinoma cell line) cells stained for Ki67 (green) using ab238020 at 2 μg/mL in ICC. The membrane is labelled with phalloidin (red).
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Protein Array - Anti-Ki67 antibody (ab238020)ab238020 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab238020 は 5 報の論文で使用されています。
- Chen Y et al. Increased ABCC2 expression predicts cisplatin resistance in non-small cell lung cancer. Cell Biochem Funct 39:277-286 (2021). PubMed: 32815556
- Bedard MC et al. Patient-Derived Organotypic Epithelial Rafts Model Phenotypes in Juvenile-Onset Recurrent Respiratory Papillomatosis. Viruses 13:N/A (2021). PubMed: 33418959
- Liu H et al. Lidocaine has antitumor effect on hepatocellular carcinoma via the circ_DYNC1H1/miR-520a-3p/USP14 axis. Open Life Sci 16:766-780 (2021). PubMed: 34435133
- He Z et al. Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression. J Exp Clin Cancer Res 39:140 (2020). PubMed: 32698859
- Lee H et al. Combinatorial Inhibition of Cell Surface Receptors Using Dual Aptamer-Functionalized Nanoconstructs for Cancer Treatment. Pharmaceutics 12:N/A (2020). PubMed: 32708267
