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Anti-Ki67 抗体 [BLR021E] - BSA free (ab272075)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
  • Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
  • Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR021E] to Ki67 - BSA free
  • Suitable for: WB, ICC, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Ki67 antibody [BLR021E] - BSA free
    Ki67 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [BLR021E] to Ki67 - BSA free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide within Human Ki67 aa 1200-1250. The exact sequence is proprietary. Corresponds to the repetitive 66 bp element. NP_002408.2 and Gene ID 4288.
    Database link: P46013

  • ポジティブ･コントロール

    • IHC-P: Human tonsil tissue. ICC/IF: OVCAR-8 cells

  • 特記事項

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272075 is the carrier-free version of ab243878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC
Human
IHC-P
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

For nuclear proteins, fixing cells in 4% PFA (20 min, room temp) is recommended.

It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen.

Positive Control: HeLa and HAP1 cells / Rt cardiomyocytes
IHC-P
Use at an assay dependent concentration.

Antigen retrieval: Heat in citrate buffer pH 6 for 20-30 min or enzymatic (trypsin, proteinase K) necessary if fixed in PFA.

Positive Control: Hu tonsil tissue, Ms - tumour, embryonic skin tissue, Rt - esophagus, small intestine and liver tissue
特記事項
WB
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

For nuclear proteins, fixing cells in 4% PFA (20 min, room temp) is recommended.

It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen.

Positive Control: HeLa and HAP1 cells / Rt cardiomyocytes
IHC-P
Use at an assay dependent concentration.

Antigen retrieval: Heat in citrate buffer pH 6 for 20-30 min or enzymatic (trypsin, proteinase K) necessary if fixed in PFA.

Positive Control: Hu tonsil tissue, Ms - tumour, embryonic skin tissue, Rt - esophagus, small intestine and liver tissue

ターゲット情報

  • 機能

    Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.

  • 配列類似性

    Contains 1 FHA domain.
    Contains 16 K167R repeats.
    Contains 1 PP1-binding domain.

  • 発生段階

    Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).

  • 翻訳後修飾

    Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

  • 細胞内局在

    Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen identified by monoclonal antibody Ki-67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • Antigen Ki67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • Marker of proliferation Ki-67 antibody
      • MIB 1 antibody
      • MIB antibody
      • MKI67 antibody
      • PPP1R105 antibody
      • Proliferation marker protein Ki-67 antibody
      • Proliferation related Ki 67 antigen antibody
      • Protein phosphatase 1 regulatory subunit 105 antibody
      • RP11-380J17.2 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab243878 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).

    • Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
      Immunocytochemistry - Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
      Paraffin-embedded, Formaldehyde-fixed OVCAR-8 cells labeling Ki67 using ab243878 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243878).

    • Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)
      Anti-Ki67 antibody [BLR021E] - BSA free (ab272075)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272075 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272075 は論文での使用が確認できていません。

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