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リコンビナント

Anti-Ki67 抗体 [BLR021E] (ab243878)

Submit a review Submit a question References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)
  • Immunocytochemistry - Anti-Ki67 antibody [BLR021E] (ab243878)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [BLR021E] (ab243878)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)
  • Flow Cytometry - Anti-Ki67 antibody [BLR021E] (ab243878)
  • Anti-Ki67 antibody [BLR021E] (ab243878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR021E] to Ki67
  • Suitable for: IHC-P, ICC, ICC/IF, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Ki67 antibody [BLR021E]
    Ki67 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [BLR021E] to Ki67

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, ICC, ICC/IF, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Ki67 aa 1200-1250. The exact sequence is proprietary. Corresponds to the repetitive 66 bp element. NP_002408.2 and Gene ID 4288.
    Database link: P46013

  • ポジティブ･コントロール

    • IHC-P: Human tonsil and ovarian carcinoma tissue. ICC: OVCAR-4 cells. ICC/IF: Acetone/methanol fixed Hela cells. Flow Cyt: MOLT4 cells.

  • 特記事項

    This product is sold under License from Bethyl Laboratories, Inc.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab243878の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/100 - 1/500.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
ICC/IF
1/100 - 1/500.
Flow Cyt
Use 1µl for 106 cells.
特記事項
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/100 - 1/500.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
ICC/IF
1/100 - 1/500.
Flow Cyt
Use 1µl for 106 cells.

ターゲット情報

  • 機能

    Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.

  • 配列類似性

    Contains 1 FHA domain.
    Contains 16 K167R repeats.
    Contains 1 PP1-binding domain.

  • 発生段階

    Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).

  • 翻訳後修飾

    Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

  • 細胞内局在

    Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen identified by monoclonal antibody Ki-67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • Antigen Ki67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • Marker of proliferation Ki-67 antibody
      • MIB 1 antibody
      • MIB antibody
      • MKI67 antibody
      • PPP1R105 antibody
      • Proliferation marker protein Ki-67 antibody
      • Proliferation related Ki 67 antigen antibody
      • Protein phosphatase 1 regulatory subunit 105 antibody
      • RP11-380J17.2 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue, labeling Ki67 with ab243878. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Detection: DAB.

    • Immunocytochemistry - Anti-Ki67 antibody [BLR021E] (ab243878)
      Immunocytochemistry - Anti-Ki67 antibody [BLR021E] (ab243878)

      Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-4 cells, labeling Ki67 with ab243878. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Detection: DAB.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [BLR021E] (ab243878)
      Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [BLR021E] (ab243878)

      Immunocytochemical analysis of acetone/menthanol fixed HeLa cells, labeling Ki67 with ab243878 at a 1/100 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Counterstain: DAPI.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue, labeling Ki67 with ab243878 at a 1/500 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody. Substrate: Opal™. Counterstain: DAPI (blue).

    • Flow Cytometry - Anti-Ki67 antibody [BLR021E] (ab243878)
      Flow Cytometry - Anti-Ki67 antibody [BLR021E] (ab243878)
      Flow cytometric analysis of 4% formaldehyde and permeabilized with 90% methanol MOLT-4 (Human lymphoblastic leukemia cell line) cells labelling Ki67 with ab243878 at 1µl/106 cells (Red shaded) compared with a Isotype control (unshaded). DyLight® 650-conjugated goat anti-rabbit IgG was used as the secondary antibody.
       
    • Anti-Ki67 antibody [BLR021E] (ab243878)
      Anti-Ki67 antibody [BLR021E] (ab243878)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab243878 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab243878 は 4 報の論文で使用されています。

    • Hu X  et al. Knockdown of SLCO4C1 inhibits cell proliferation and metastasis in endometrial cancer through inactivating the PI3K/Akt signaling pathway. Oncol Rep 43:919-929 (2020). PubMed: 32020231
    • Dou D  et al. CircUBE2D2 (hsa_circ_0005728) promotes cell proliferation, metastasis and chemoresistance in triple-negative breast cancer by regulating miR-512-3p/CDCA3 axis. Cancer Cell Int 20:454 (2020). PubMed: 32944002
    • Chen L  et al. Silencing transcription factor FOXM1 represses proliferation, migration, and invasion while inducing apoptosis of liver cancer stem cells by regulating the expression of ALDH2. IUBMB Life 72:285-295 (2020). PubMed: 31580537
    • Yan C  et al. LncRNA HULC shRNA disinhibits miR-377-5p to suppress the growth and invasion of hepatocellular carcinoma in vitro and hepatocarcinogenesis in vivo. Ann Transl Med 8:1294 (2020). PubMed: 33209874

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