Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Specification
PBMCs
Preparation
Cell harvesting/tissue preparation method: Ficoll density separation
Sample buffer: PBS/ 1%FCS/ 0,09% sodium azide
Sample buffer: PBS/ 1%FCS/ 0,09% sodium azide
Fixation
Paraformaldehyde
Permeabilization
Yes - 0,1% Saponin / PBS
Gating Strategy
Lymphocytes and Monocytes
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/ 1%FCS/ 0,09% sodium azide
Secondary antibody
Name
Non-Abcam antibody was used: goat-anti-rabbit PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100
Additional data
Additional Notes
picture:
1 = isotype;
2 = PBMCs untreated;
3 = PBMCs treated with PHA.
1 = isotype;
2 = PBMCs untreated;
3 = PBMCs treated with PHA.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 11 2012