ketohexokinase 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to ketohexokinase
アプリケーション適用あり: IHC-P, ELISA, WB, ICC/IFmore details
種交差性交差種: Mouse, Human
Synthetic peptide conjugated to KLH selected within the last 50 aa of human ketohexokinase.
- Mouse kidney tissue lysate. IHC-P: FFPE human kidney tissue sections. IF/ICC: HeLa cell line.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
バッファーPreservative: 0.09% Sodium azide
Concentration information loading...
精製度Protein G purified
特記事項（精製）This antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab38281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 33 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
組織特異性Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
パスウェイCarbohydrate metabolism; fructose metabolism.
関連疾患Defects in KHK are the cause of fructosuria (FRUCT) [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism.
配列類似性Belongs to the carbohydrate kinase pfkB family.
- Information by UniProt
- EC 22.214.171.124 antibody
- Fructokinase antibody
- Hepatic fructokinase antibody
Anti-ketohexokinase antibody (ab38281) at 1/100 dilution + Mouse kidney tissue lysate
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab38281 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38281, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ketohexokinase staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38281, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Palavicino-Maggio CB & Kuzhikandathil EV Dietary Fructose and GLUT5 Transporter Activity Contribute to Antipsychotic-Induced Weight Gain. Schizophr Bull 42:1270-9 (2016). Read more (PubMed: 27056716) »
- Della Casa L et al. Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice. Genes Nutr 11:26 (2016). Read more (PubMed: 27713773) »