Anti-KDM4C / GASC1 / JMJD2C 抗体 (ab85454)


  • 製品名
    Anti-KDM4C / GASC1 / JMJD2C antibody
    KDM4C / GASC1 / JMJD2C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KDM4C / GASC1 / JMJD2C
  • 由来種
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 755-804 (CLCNLRGGAL KQTKNNKWAH VMCAVAVPEV RFTNVPERTQ IDVGRIPLQR) of Human KDM4C/ GASC1/ JMJD2C, NP_055876.

  • ポジティブ・コントロール
    • HepG2 cell lysate



Our Abpromise guarantee covers the use of ab85454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 120 kDa).Can be blocked with Human KDM4C / GASC1 / JMJD2C peptide (ab111662) or KDM4C / GASC1 / JMJD2C peptide (ab155886). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 機能
    Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • 組織特異性
    Overexpressed in several esophageal squamous cell carcinomas (ESCs).
  • 配列類似性
    Belongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • ドメイン
    The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
  • 細胞内局在
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA146B14.1 antibody
    • GASC 1 protein antibody
    • GASC-1 protein antibody
    • GASC1 antibody
    • Gene amplified in squamous cell carcinoma 1 protein antibody
    • JHDM3C antibody
    • JmjC domain containing histone demethylation protein 3C antibody
    • JmjC domain-containing histone demethylation protein 3C antibody
    • JMJD2C antibody
    • Jumonji domain containing 2C antibody
    • Jumonji domain containing protein 2C antibody
    • Jumonji domain-containing protein 2C antibody
    • Kdm4c antibody
    • KDM4C_HUMAN antibody
    • Lysine (K) specific demethylase 4C antibody
    • Lysine demethylase 4C antibody
    • Lysine-specific demethylase 4C antibody
    • TDRD14C antibody
    • Tudor domain containing 14C antibody
    see all


  • Anti-KDM4C / GASC1 / JMJD2C antibody (ab85454) at 1 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 120 kDa
    Observed band size: 100 kDa (why is the actual band size different from the predicted?)

    Gel concentration 6-18%


This product has been referenced in:
  • Yu Y  et al. Targeting the Senescence-Overriding Cooperative Activity of Structurally Unrelated H3K9 Demethylases in Melanoma. Cancer Cell 33:322-336.e8 (2018). Read more (PubMed: 29438700) »
  • Brügger V  et al. Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Nat Commun 8:14272 (2017). IF . Read more (PubMed: 28139683) »

See all 5 Publications for this product

レビューと Q&A

I have a follow up question based on multiple bands disappearing when the blocking peptide is added. What are the molecular weights of the other bands in your blot? If more than one band disappears in Western blot by peptide/antigen competition, those ...

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Thank you for your reply.

I have sent you the blocking peptide ( free of charge. Your order number is 1236828, and is expected to be delivered Friday, 1/18/2013.

Below i...

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Knockdown at the mRNA level does not necessarily equate to a visible difference at the protein level in the WB band intensity. Since 100kDa is the expected band size for KDM4C, it is very likely that this band is not non-specific but rather a refl...

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Vielen Dank für Ihre Antwort.

Die Referenznummer Ihrer Gutschrift ist 123456

Bitte setzen Sie sich mit der Apotheke/Reagenzienzentrale in Kontakt, um zu verhindern, dass diese Ihre Gutschrift/die Erstattung ohne Ihr Einverst&a...

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Leider hat sich mein Verdacht bestätigt: der Antikörper wurde noch nicht in der Maus getestet. ich habe unser Datenblatt dementsprechend korrigiert.

Es ist übrigens sehr interessant, dass der Antikörper nicht kreuzreagiert, da die beiden Pro...

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I am very pleased to hear you would like to accept our offer and test these antibodies noted above in IP. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please sub...

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I am sorry to hear you are disappointed with our products. I appreciate the time you have spent on these experiments in the laboratory and it ...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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I have checked our system which stores all the incoming and outgoing e-mails and I can see that in the initial correspondence, 3 products (ab47535, ab71732 and ab85454) were reported to be faulty.

Could ...

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My colleague is out of office and she has asked me to look after her customers. I could arrange a credit note for you which you can use in the future.

Please do let me know how you wish to proceed with y...

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