アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    kdm2b-antibody-ab5199.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
Share by email

Anti-KDM2B 抗体 (ab5199)

  • Datasheet
Reviews (2)Q&A (1)References (8)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Goat polyclonal to KDM2B
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
タンパク質
Product image
Recombinant Human KDM2B protein (ab164635)
一次抗体
Product image
Anti-EZH2 antibody [11/EZH2] (ab283270)

関連製品

製品の概要

  • 製品名

    Anti-KDM2B antibody
    KDM2B 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to KDM2B
  • 由来種

    Goat
  • アプリケーション

    適用あり: IHC-Pmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Dictyostelium discoideum
  • 免疫原

    Synthetic peptide corresponding to Human KDM2B aa 1324-1336.
    Sequence:

    C-FGQVEEKLLQKLS


    Database link: Q8NHM5
    (Peptide available as ab23079)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • F-box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human KDM2B protein (ab164635)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5199の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
IHC-P
Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
  • 配列類似性

    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.
  • ドメイン

    The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.
  • 細胞内局在

    Nucleus > nucleolus.
  • Target information above from: UniProt accession Q8NHM5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 84678 Human
    • Omim: 609078 Human
    • SwissProt: Q8NHM5 Human
    • Unigene: 524800 Human
    • 別名

      • [Histone-H3]-lysine-36 demethylase 1B antibody
      • CXXC-type zinc finger protein 2 antibody
      • CXXC2 antibody
      • F box and leucine rich repeat protein 10 antibody
      • F box protein FBL10 antibody
      • F-box and leucine-rich repeat protein 10 antibody
      • F-box protein FBL10 antibody
      • F-box/LRR-repeat protein 10 antibody
      • Fbl10 antibody
      • FBXL10 antibody
      • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
      • JHDM1B antibody
      • JmjC domain-containing histone demethylation protein 1B antibody
      • Jumonji C domain containing histone demethylase 1B antibody
      • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
      • kdm2b antibody
      • KDM2B_HUMAN antibody
      • Lysine (K) specific demethylase 2B antibody
      • Lysine-specific demethylase 2B antibody
      • PCCX2 antibody
      • Protein containing CXXC domain 2 antibody
      • Protein JEMMA antibody
      • Protein-containing CXXC domain 2 antibody
      see all

    プロトコール

    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (8)

    ab5199 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5199 は 8 報の論文で使用されています。

    • Saha T  et al. Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner. Biochem Biophys Rep 25:100912 (2021). PubMed: 33537462
    • Vargas-Ayala RC  et al. Interplay between the Epigenetic Enzyme Lysine (K)-Specific Demethylase 2B and Epstein-Barr Virus Infection. J Virol 93:N/A (2019). PubMed: 30996097
    • Yan M  et al. miR-146b promotes cell proliferation and increases chemosensitivity, but attenuates cell migration and invasion via FBXL10 in ovarian cancer. Cell Death Dis 9:1123 (2018). PubMed: 30409964
    • Zhang DD  et al. Vildagliptin, a DPP4 inhibitor, alleviates diabetes-associated cognitive deficits by decreasing the levels of apoptosis-related proteins in the rat hippocampus. Exp Ther Med 15:5100-5106 (2018). PubMed: 29805536
    • He P  et al. The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol 311:F343-51 (2016). PubMed: 27279487
    • He P  et al. Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss. J Clin Invest 125:3519-31 (2015). PubMed: 26258413
    • Tzatsos A  et al. Lysine-specific Demethylase 2B (KDM2B)-let-7-Enhancer of Zester Homolog 2 (EZH2) Pathway Regulates Cell Cycle Progression and Senescence in Primary Cells. J Biol Chem 286:33061-9 (2011). WB ; Mouse . PubMed: 21757686
    • Ge R  et al. F-box protein 10, an NF-?B-dependent anti-apoptotic protein, regulates TRAIL-induced apoptosis through modulating c-Fos/c-FLIP pathway. Cell Death Differ 18:1184-95 (2011). PubMed: 21252908

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-KDM2B antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (NIH3T3, Neuro2A and ES)
    Loading amount
    20 µg
    Specification
    NIH3T3, Neuro2A and ES
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 28 2010

    Western blot abreview for Anti-KDM2B antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    100 µg
    Specification
    HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 26 2006

    Question

    LOT NUMBER 626682 ORDER NUMBER 2 (dated 05/01/2011) DESCRIPTION OF THE PROBLEM High background, more bands SAMPLE MCF-7 and ZR-75-1 human cell lines whole cell protein extracts PRIMARY ANTIBODY Abcam Goat anti JHDM1B diluted 1/1000 or 1/2000 in TBS-Tween + 3.5% BSA, or 2% blocking agent as above in TBS-T, or 2% Normal Goat serum in TBS-T, O/N @ 4°C, followed by 3 washes of 10' each in TBS-Tween at RT DETECTION METHOD ECL Advance from GE healthcare POSITIVE AND NEGATIVE CONTROLS USED None ANTIBODY STORAGE CONDITIONS -20°C as specified by spec sheet SAMPLE PREPARATION Proteins extracted in: Igepal 1% KH2PO4 0.1M pH7.5 0.1mM Glycerophosphate (standard buffer in our lab) added protease inhibitors mix added Laemli loading buffer and heated for 10' 95°C AMOUNT OF PROTEIN LOADED 15 micrograms ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 6%, run through stacking at 50V and through resolving at 150V Standard TGS Running buffer TRANSFER AND BLOCKING CONDITIONS Standard Tris/Glycine/SDS transfer buffer, 280mA 2Hours 4°C. Blocking agent: tried 2% normal goat serum in TBS-Tween 1h @ RT or 2% GE Health Care Advance ECL Kit Blocking agent in TBS-Tween (as recommended by manufacturer: 1h @ RT) SECONDARY ANTIBODY Santa Cruz donkey anti Goat dilutent: as above for primary antibody 1/2500, incubation 1h @ RT, followed by 3 washes of 10' each in TBS-Tween at RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? Blocking conditions and primary/secondary antibody dilution and incubation conditions

    Read More

    Abcam community

    Verified customer

    Asked on Nov 10 2011

    Answer

    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions: 1) Isoform: It is important to consider that at least 3 isoforms (Q8NHM5, Q8NHM5-2, Q8NHM5-3) have been described which may run differently on the gel. 2) Sample preparation: KDM2B is expressed mainly in the nucleoli, so it may be worth preparing nuclear lysate (rather than whole cell lysate) for Western blot application. 3) Positive control: This antibody has been tested on cell cell lyaste of HeLa, so running this type of lysate alongside with the samples - as positive control - may be useful. 4) Detections system: Does the detection system work fine? Have you used it successfully with another primary antibody? Have you run a no primary - only secondary antibody - control to see if any of the non-specific bands are due to the secondary or not? If you have not done yet, I would advise you to check it. I hope this will be useful for you.

    Read More

    Abcam Scientific Support

    Answered on Nov 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.