Anti-KDM2B 抗体 (ab5199)
Key features and details
- Goat polyclonal to KDM2B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KDM2B antibody
KDM2B 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to KDM2B -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Dictyostelium discoideum -
免疫原
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-P | Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. -
配列類似性
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
ドメイン
The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 84678 Human
- Omim: 609078 Human
- SwissProt: Q8NHM5 Human
- Unigene: 524800 Human
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別名
- [Histone-H3]-lysine-36 demethylase 1B antibody
- CXXC-type zinc finger protein 2 antibody
- CXXC2 antibody
see all
データシートおよび資料
参考文献 (7)
ab5199 は 7 報の論文で使用されています。
- Vargas-Ayala RC et al. Interplay between the Epigenetic Enzyme Lysine (K)-Specific Demethylase 2B and Epstein-Barr Virus Infection. J Virol 93:N/A (2019). PubMed: 30996097
- Yan M et al. miR-146b promotes cell proliferation and increases chemosensitivity, but attenuates cell migration and invasion via FBXL10 in ovarian cancer. Cell Death Dis 9:1123 (2018). PubMed: 30409964
- Zhang DD et al. Vildagliptin, a DPP4 inhibitor, alleviates diabetes-associated cognitive deficits by decreasing the levels of apoptosis-related proteins in the rat hippocampus. Exp Ther Med 15:5100-5106 (2018). PubMed: 29805536
- He P et al. The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol 311:F343-51 (2016). PubMed: 27279487
- He P et al. Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss. J Clin Invest 125:3519-31 (2015). PubMed: 26258413
- Tzatsos A et al. Lysine-specific Demethylase 2B (KDM2B)-let-7-Enhancer of Zester Homolog 2 (EZH2) Pathway Regulates Cell Cycle Progression and Senescence in Primary Cells. J Biol Chem 286:33061-9 (2011). WB ; Mouse . PubMed: 21757686
- Ge R et al. F-box protein 10, an NF-?B-dependent anti-apoptotic protein, regulates TRAIL-induced apoptosis through modulating c-Fos/c-FLIP pathway. Cell Death Differ 18:1184-95 (2011). PubMed: 21252908