Anti-KDM2B 抗体 (ab5199)

Reviews (2)Q&A (1)References (6)

Key features and details

  • Goat polyclonal to KDM2B
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

  • 配列類似性

    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.

  • ドメイン

    The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.

  • 細胞内局在

    Nucleus > nucleolus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • [Histone-H3]-lysine-36 demethylase 1B antibody
      • CXXC-type zinc finger protein 2 antibody
      • CXXC2 antibody
      • F box and leucine rich repeat protein 10 antibody
      • F box protein FBL10 antibody
      • F-box and leucine-rich repeat protein 10 antibody
      • F-box protein FBL10 antibody
      • F-box/LRR-repeat protein 10 antibody
      • Fbl10 antibody
      • FBXL10 antibody
      • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
      • JHDM1B antibody
      • JmjC domain-containing histone demethylation protein 1B antibody
      • Jumonji C domain containing histone demethylase 1B antibody
      • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
      • kdm2b antibody
      • KDM2B_HUMAN antibody
      • Lysine (K) specific demethylase 2B antibody
      • Lysine-specific demethylase 2B antibody
      • PCCX2 antibody
      • Protein containing CXXC domain 2 antibody
      • Protein JEMMA antibody
      • Protein-containing CXXC domain 2 antibody
      see all

    参考文献 (6)

    ab5199 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5199 は 6 報の論文で使用されています。

    • Zhang DD  et al. Vildagliptin, a DPP4 inhibitor, alleviates diabetes-associated cognitive deficits by decreasing the levels of apoptosis-related proteins in the rat hippocampus. Exp Ther Med 15:5100-5106 (2018). PubMed: 29805536
    • Yan M  et al. miR-146b promotes cell proliferation and increases chemosensitivity, but attenuates cell migration and invasion via FBXL10 in ovarian cancer. Cell Death Dis 9:1123 (2018). PubMed: 30409964
    • He P  et al. The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol 311:F343-51 (2016). PubMed: 27279487
    • He P  et al. Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss. J Clin Invest 125:3519-31 (2015). PubMed: 26258413
    • Tzatsos A  et al. Lysine-specific Demethylase 2B (KDM2B)-let-7-Enhancer of Zester Homolog 2 (EZH2) Pathway Regulates Cell Cycle Progression and Senescence in Primary Cells. J Biol Chem 286:33061-9 (2011). WB ; Mouse . PubMed: 21757686
    • Ge R  et al. F-box protein 10, an NF-?B-dependent anti-apoptotic protein, regulates TRAIL-induced apoptosis through modulating c-Fos/c-FLIP pathway. Cell Death Differ 18:1184-95 (2011). PubMed: 21252908

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    LOT NUMBER 626682 ORDER NUMBER 2 (dated 05/01/2011) DESCRIPTION OF THE PROBLEM High background, more bands SAMPLE MCF-7 and ZR-75-1 human cell lines whole cell protein extracts PRIMARY ANTIBODY Abcam Goat anti JHDM1B diluted 1/1000 or 1/2000 in TBS-Tween + 3.5% BSA, or 2% blocking agent as above in TBS-T, or 2% Normal Goat serum in TBS-T, O/N @ 4°C, followed by 3 washes of 10' each in TBS-Tween at RT DETECTION METHOD ECL Advance from GE healthcare POSITIVE AND NEGATIVE CONTROLS USED None ANTIBODY STORAGE CONDITIONS -20°C as specified by spec sheet SAMPLE PREPARATION Proteins extracted in: Igepal 1% KH2PO4 0.1M pH7.5 0.1mM Glycerophosphate (standard buffer in our lab) added protease inhibitors mix added Laemli loading buffer and heated for 10' 95°C AMOUNT OF PROTEIN LOADED 15 micrograms ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 6%, run through stacking at 50V and through resolving at 150V Standard TGS Running buffer TRANSFER AND BLOCKING CONDITIONS Standard Tris/Glycine/SDS transfer buffer, 280mA 2Hours 4°C. Blocking agent: tried 2% normal goat serum in TBS-Tween 1h @ RT or 2% GE Health Care Advance ECL Kit Blocking agent in TBS-Tween (as recommended by manufacturer: 1h @ RT) SECONDARY ANTIBODY Santa Cruz donkey anti Goat dilutent: as above for primary antibody 1/2500, incubation 1h @ RT, followed by 3 washes of 10' each in TBS-Tween at RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? Blocking conditions and primary/secondary antibody dilution and incubation conditions

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    Answer

    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions: 1) Isoform: It is important to consider that at least 3 isoforms (Q8NHM5, Q8NHM5-2, Q8NHM5-3) have been described which may run differently on the gel. 2) Sample preparation: KDM2B is expressed mainly in the nucleoli, so it may be worth preparing nuclear lysate (rather than whole cell lysate) for Western blot application. 3) Positive control: This antibody has been tested on cell cell lyaste of HeLa, so running this type of lysate alongside with the samples - as positive control - may be useful. 4) Detections system: Does the detection system work fine? Have you used it successfully with another primary antibody? Have you run a no primary - only secondary antibody - control to see if any of the non-specific bands are due to the secondary or not? If you have not done yet, I would advise you to check it. I hope this will be useful for you.

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    Western blot abreview for Anti-KDM2B antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (NIH3T3, Neuro2A and ES)
    Loading amount
    20 µg
    Specification
    NIH3T3, Neuro2A and ES
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 28 2010

    Western blot abreview for Anti-KDM2B antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    100 µg
    Specification
    HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 26 2006

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