Anti-KDM2B 抗体 (ab5199)

Reviews (2)Q&A (1)References (7)

Key features and details

  • Goat polyclonal to KDM2B
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-KDM2B antibody
    KDM2B 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to KDM2B

  • 由来種

    Goat

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Dictyostelium discoideum

  • 免疫原

    Synthetic peptide corresponding to Human KDM2B aa 1324-1336.
    Sequence:

    C-FGQVEEKLLQKLS


    Database link: Q8NHM5
    (Peptide available as ab23079)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

  • 配列類似性

    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.

  • ドメイン

    The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.

  • 細胞内局在

    Nucleus > nucleolus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • [Histone-H3]-lysine-36 demethylase 1B antibody
      • CXXC-type zinc finger protein 2 antibody
      • CXXC2 antibody
      • F box and leucine rich repeat protein 10 antibody
      • F box protein FBL10 antibody
      • F-box and leucine-rich repeat protein 10 antibody
      • F-box protein FBL10 antibody
      • F-box/LRR-repeat protein 10 antibody
      • Fbl10 antibody
      • FBXL10 antibody
      • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
      • JHDM1B antibody
      • JmjC domain-containing histone demethylation protein 1B antibody
      • Jumonji C domain containing histone demethylase 1B antibody
      • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
      • kdm2b antibody
      • KDM2B_HUMAN antibody
      • Lysine (K) specific demethylase 2B antibody
      • Lysine-specific demethylase 2B antibody
      • PCCX2 antibody
      • Protein containing CXXC domain 2 antibody
      • Protein JEMMA antibody
      • Protein-containing CXXC domain 2 antibody
      see all

    参考文献 (7)

    ab5199 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5199 は 7 報の論文で使用されています。

    • Vargas-Ayala RC  et al. Interplay between the Epigenetic Enzyme Lysine (K)-Specific Demethylase 2B and Epstein-Barr Virus Infection. J Virol 93:N/A (2019). PubMed: 30996097
    • Yan M  et al. miR-146b promotes cell proliferation and increases chemosensitivity, but attenuates cell migration and invasion via FBXL10 in ovarian cancer. Cell Death Dis 9:1123 (2018). PubMed: 30409964
    • Zhang DD  et al. Vildagliptin, a DPP4 inhibitor, alleviates diabetes-associated cognitive deficits by decreasing the levels of apoptosis-related proteins in the rat hippocampus. Exp Ther Med 15:5100-5106 (2018). PubMed: 29805536
    • He P  et al. The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol 311:F343-51 (2016). PubMed: 27279487
    • He P  et al. Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss. J Clin Invest 125:3519-31 (2015). PubMed: 26258413
    • Tzatsos A  et al. Lysine-specific Demethylase 2B (KDM2B)-let-7-Enhancer of Zester Homolog 2 (EZH2) Pathway Regulates Cell Cycle Progression and Senescence in Primary Cells. J Biol Chem 286:33061-9 (2011). WB ; Mouse . PubMed: 21757686
    • Ge R  et al. F-box protein 10, an NF-?B-dependent anti-apoptotic protein, regulates TRAIL-induced apoptosis through modulating c-Fos/c-FLIP pathway. Cell Death Differ 18:1184-95 (2011). PubMed: 21252908

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    LOT NUMBER 626682 ORDER NUMBER 2 (dated 05/01/2011) DESCRIPTION OF THE PROBLEM High background, more bands SAMPLE MCF-7 and ZR-75-1 human cell lines whole cell protein extracts PRIMARY ANTIBODY Abcam Goat anti JHDM1B diluted 1/1000 or 1/2000 in TBS-Tween + 3.5% BSA, or 2% blocking agent as above in TBS-T, or 2% Normal Goat serum in TBS-T, O/N @ 4°C, followed by 3 washes of 10' each in TBS-Tween at RT DETECTION METHOD ECL Advance from GE healthcare POSITIVE AND NEGATIVE CONTROLS USED None ANTIBODY STORAGE CONDITIONS -20°C as specified by spec sheet SAMPLE PREPARATION Proteins extracted in: Igepal 1% KH2PO4 0.1M pH7.5 0.1mM Glycerophosphate (standard buffer in our lab) added protease inhibitors mix added Laemli loading buffer and heated for 10' 95°C AMOUNT OF PROTEIN LOADED 15 micrograms ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 6%, run through stacking at 50V and through resolving at 150V Standard TGS Running buffer TRANSFER AND BLOCKING CONDITIONS Standard Tris/Glycine/SDS transfer buffer, 280mA 2Hours 4°C. Blocking agent: tried 2% normal goat serum in TBS-Tween 1h @ RT or 2% GE Health Care Advance ECL Kit Blocking agent in TBS-Tween (as recommended by manufacturer: 1h @ RT) SECONDARY ANTIBODY Santa Cruz donkey anti Goat dilutent: as above for primary antibody 1/2500, incubation 1h @ RT, followed by 3 washes of 10' each in TBS-Tween at RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? Blocking conditions and primary/secondary antibody dilution and incubation conditions

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    Answer

    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. After reading through the detailed protocol you kindly forwarded to Abcam, I would like to make the following comments/suggestions: 1) Isoform: It is important to consider that at least 3 isoforms (Q8NHM5, Q8NHM5-2, Q8NHM5-3) have been described which may run differently on the gel. 2) Sample preparation: KDM2B is expressed mainly in the nucleoli, so it may be worth preparing nuclear lysate (rather than whole cell lysate) for Western blot application. 3) Positive control: This antibody has been tested on cell cell lyaste of HeLa, so running this type of lysate alongside with the samples - as positive control - may be useful. 4) Detections system: Does the detection system work fine? Have you used it successfully with another primary antibody? Have you run a no primary - only secondary antibody - control to see if any of the non-specific bands are due to the secondary or not? If you have not done yet, I would advise you to check it. I hope this will be useful for you.

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    Western blot abreview for Anti-KDM2B antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (NIH3T3, Neuro2A and ES)
    Loading amount
    20 µg
    Specification
    NIH3T3, Neuro2A and ES
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 28 2010

    Western blot abreview for Anti-KDM2B antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    100 µg
    Specification
    HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 26 2006

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