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  1. Link

    kdel-antibody-ab2898.pdf

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Tags & Cell Markers Subcellular Markers Organelles ER
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Anti-KDEL 抗体 (ab2898)

  • Datasheet
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Abpromise

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Flow Cytometry - Anti-KDEL antibody (ab2898)
  • Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (ab2898)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)

Key features and details

  • Rabbit polyclonal to KDEL
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-KDEL antibody
    KDEL 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to KDEL
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Rat KDEL aa 643-654.
    Sequence:

    TGEEDTSEKDEL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • IHC: Mouse lymph node, pancreas and liver tissues. ICC: human U251 cells Flow Cyt: human HeLa cells
  • 特記事項

    This antibody can be used as an endoplasmic reticulum (ER) marker.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2898の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use a concentration of 1 - 20 µg/ml.
ICC/IF
Use at an assay dependent concentration.
IHC-P (1)
1/100 - 1/200.
特記事項
Flow Cyt
Use a concentration of 1 - 20 µg/ml.
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/100 - 1/200.

ターゲット情報

  • 関連性

    The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
  • 細胞内局在

    Endoplasmic reticulum
  • 別名

    • KDEL antibody
    • Lys Asp Glu Leu antibody

画像

  • Flow Cytometry - Anti-KDEL antibody (ab2898)
    Flow Cytometry - Anti-KDEL antibody (ab2898)

    Flow cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KDEL with ab2898 (purple) or a rabbit IgG isotype control (black) at a 10 µg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 647 conjugate at  1/50 dilution for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

  • Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (ab2898)
    Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody (ab2898)

    Immunofluorescent analysis of U-251 MG (Human brain glioma cell line) cells labeling KDEL (green) with ab2898. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2898 at 1/200 dilution overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody (ab2898)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse pancreas tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing KDEL ab2898 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

プロトコール

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (3)

ab2898 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2898 は 3 報の論文で使用されています。

  • Wang H  et al. N-Glycan-calnexin interactions in human factor VII secretion and deficiency. Int J Biochem Cell Biol 113:67-74 (2019). PubMed: 31185295
  • Schröder PC  et al. Proteomic analysis of human hepatoma cells expressing methionine adenosyltransferase I/III: Characterization of DDX3X as a target of S-adenosylmethionine. J Proteomics 75:2855-68 (2012). PubMed: 22270009
  • Sánchez-Quiles V  et al. HSV-1 Cgal+ infection promotes quaking RNA binding protein production and induces nuclear-cytoplasmic shuttling of quaking I-5 isoform in human hepatoma cells. Mol Cell Proteomics 10:M111.009126 (2011). WB . PubMed: 21467216

レビューと Q&A

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KDEL antibody - ER Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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投稿 Feb 07 2011

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