Application
Western blot
Sample
Pig Tissue lysate - whole (Spinal cord)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Spinal cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocker
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate #1
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 180 kDa
Additional data
Additional Notes
Lane 1: 30 ug Spinal Cord Tissue Lysate
Lane 2: 90 ug Spinal Cord Tissue Lysate
Membrane: Nitrocellulose 0.2 um
Transfer buffer: Towbin Buffer 0.035% SDS, 20% Methanol
Transfer conditions: 30 mA overnight.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 19 2022