Anti-KAT7 / Hbo1 / MYST2 抗体 [EPR7194(B)] - BSA and Azide free (ab248087)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7194(B)] to KAT7 / Hbo1 / MYST2 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free
KAT7 / Hbo1 / MYST2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7194(B)] to KAT7 / Hbo1 / MYST2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248087 is the carrier-free version of ab124993.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 4.70 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7194(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248087の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription. -
組織特異性
Ubiquitously expressed, with highest levels in testis. -
配列類似性
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger. -
ドメイン
The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 11143 Human
- Entrez Gene: 217127 Mouse
- Entrez Gene: 303470 Rat
- Omim: 609880 Human
- SwissProt: O95251 Human
- SwissProt: Q5SVQ0 Mouse
- SwissProt: Q810T5 Rat
- Unigene: 21907 Human
see all -
別名
- Hbo 1 antibody
- HBO1 antibody
- HBOa antibody
see all
画像
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All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 71 kDaThis data was developed using ab124993, the same antibody clone in a different buffer formulation.
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This data was developed using ab124993, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248087 は論文での使用が確認できていません。