Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (6% Acrylamide)
Loading amount
25 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST, 3% BSA or Milk
Dilution
1/100
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Lumiglo
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 300 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 10 2020