Application
Western blot
Sample
Chicken Tissue lysate - whole (brain)
Loading amount
5.2 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: polyclonal Secondary Antibody anti-Rabbit
Host species: Dogfish/Catshark
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Dogfish/Catshark
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
HRP
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 265 kDa Non-specific: 80, 160 kDa
Positive control
H3K27Me3 is used as positive control.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Ying Jiang
Verified customer
投稿 Sep 21 2012