Anti-KAT2B / PCAF 抗体 (ab96510)
Key features and details
- Rabbit polyclonal to KAT2B / PCAF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KAT2B / PCAF antibody
KAT2B / PCAF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KAT2B / PCAF -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- 293T, A431, H1299, HeLa S3, HepG2, MOLT4, Raji cells, HeLa cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96510の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 93 kDa. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. -
組織特異性
Ubiquitously expressed but most abundant in heart and skeletal muscle. -
配列類似性
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
ドメイン
The bromodomain mediates binding to HIV-1 Tat. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8850 Human
- Entrez Gene: 18519 Mouse
- Omim: 602303 Human
- SwissProt: Q92831 Human
- SwissProt: Q9JHD1 Mouse
- Unigene: 533055 Human
- Unigene: 255025 Mouse
- Unigene: 483572 Mouse
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別名
- CAF antibody
- CREBBP associated factor antibody
- GCN5 antibody
see all
画像
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Western blot - Anti-KAT2B / PCAF antibody (ab96510)Anti-KAT2B / PCAF antibody (ab96510) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KAT2B / PCAF antibody (ab96510)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96510 at 1:200 dilution. Image 2: Merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab96510 は 2 報の論文で使用されています。
- Shirakawa K et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . PubMed: 27244239
- Rabhi N et al. KAT2B Is Required for Pancreatic Beta Cell Adaptation to Metabolic Stress by Controlling the Unfolded Protein Response. Cell Rep 15:1051-1061 (2016). PubMed: 27117420