Anti-KAT13D / CLOCK 抗体 (ab93804)
Key features and details
- Rabbit polyclonal to KAT13D / CLOCK
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KAT13D / CLOCK antibody
KAT13D / CLOCK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KAT13D / CLOCK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, 293T and NIH3T3 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab93804 was affinity purified using an epitope specific to KAT13D/CLOCK immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
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IP |
Use a concentration of 2 - 5 µg/ml.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 95 kDa. |
IP
Use a concentration of 2 - 5 µg/ml. |
ターゲット情報
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機能
ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL. -
組織特異性
Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻訳後修飾
Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation. -
細胞内局在
Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9575 Human
- Entrez Gene: 12753 Mouse
- Entrez Gene: 100172630 Orangutan
- Omim: 601851 Human
- SwissProt: O15516 Human
- SwissProt: O08785 Mouse
- SwissProt: Q5RAK8 Orangutan
- Unigene: 436975 Human
see all -
別名
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
see all
画像
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All lanes : Anti-KAT13D / CLOCK antibody (ab93804) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CLOCK CRISPR-Cas9 edited HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : U-251 MG cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-KAT13D / CLOCK antibody staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab93804 was shown to bind specifically to KAT13D / CLOCK. A band was observed at 100 kDa in wild-type HeLa cell lysates with no signal observed at this size in CLOCK CRISPR-Cas9 edited cell line ab266054 (CRISPR-Cas9 edited cell lysate ab258365). The band observed in the CRISPR-Cas9 edited lysate lane below 100 kDa is likely to represent a truncated form of KAT13D / CLOCK. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and CLOCK CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-KAT13D / CLOCK antibody (ab93804) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CLOCK CRISPR-Cas9 edited HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : U-251 MG cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-KAT13D / CLOCK antibody staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab93804 was shown to bind specifically to KAT13D / CLOCK. A band was observed at 100 kDa in wild-type HeLa cell lysates with no signal observed at this size in CLOCK CRISPR-Cas9 edited cell line ab265301 (CRISPR-Cas9 edited cell lysate ab258364). The band observed in the CRISPR-Cas9 edited lysate lane below 100 kDa is likely to represent a truncated form of KAT13D / CLOCK. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and CLOCK CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-KAT13D / CLOCK antibody (ab93804) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 95 kDa
Exposure time: 30 secondsDetection: Chemiluminescence
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ab93804 at 1 µg/ml detecting KAT13D/CLOCK in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of KAT13D/CLOCK
Lane 2: ab93804 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab93804 は 4 報の論文で使用されています。
- Li L et al. Circadian clock gene Clock-Bmal1 regulates cellular senescence in Chronic obstructive pulmonary disease. BMC Pulm Med 22:435 (2022). PubMed: 36419003
- Li J et al. Resveratrol Maintains Lipid Metabolism Homeostasis via One of the Mechanisms Associated with the Key Circadian Regulator Bmal1. Molecules 24:N/A (2019). PubMed: 31408938
- Qi G et al. Nobiletin protects against insulin resistance and disorders of lipid metabolism by reprogramming of circadian clock in hepatocytes. Biochim Biophys Acta 1863:549-562 (2018). WB . PubMed: 29501626
- Qi G et al. Tea polyphenols direct Bmal1-driven ameliorating of the redox imbalance and mitochondrial dysfunction in hepatocytes. Food Chem Toxicol 122:181-193 (2018). PubMed: 30316844