Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Other product details
Dilution
1/2000
Incubation time
72 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: biotin-labeled goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
It seems that the shorter incubation may give negative staining, in fact we incubated 3 nights for the primary antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Jiqiang Zhang
Verified customer
投稿 May 23 2007