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  1. Link

    kat13a--src1-antibody-ab2859.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-KAT13A / SRC1 抗体 (ab2859)

  • Datasheet
Reviews (5)Q&A (1)References (6)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)
  • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
  • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
  • Flow Cytometry - Anti-KAT13A / SRC1 antibody (ab2859)

Key features and details

  • Rabbit polyclonal to KAT13A / SRC1
  • Suitable for: ICC, IHC-Fr, Flow Cyt
  • Reacts with: Mouse, Rat, Chicken, Human, Bird
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Recombinant Human KAT13A / SRC1 protein (ab81791)

関連製品

製品の概要

  • 製品名

    Anti-KAT13A / SRC1 antibody
    KAT13A / SRC1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to KAT13A / SRC1
  • 由来種

    Rabbit
  • 特異性

    Detects steroid receptor coactivator-1 (SRC-1).
  • アプリケーション

    適用あり: ICC, IHC-Fr, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Chicken, Human, Bird
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide corresponding to Human KAT13A/ SRC1 aa 8-24.
    Sequence:

    SSDPANPDSHKRKGSPC


    Database link: Q15788
    (Peptide available as ab5848)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human KAT13A / SRC1 peptide (ab5848)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human KAT13A / SRC1 protein (ab81791)

アプリケーション

Our Abpromise guarantee covers the use of ab2859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IHC-Fr Use a concentration of 1 µg/ml.
Flow Cyt Use 3-5µg for 106 cells.

ターゲット情報

  • 機能

    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • 組織特異性

    Widely expressed.
  • 関連疾患

    Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • 配列類似性

    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • ドメイン

    The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
    Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
  • 翻訳後修飾

    Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
    Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession Q15788 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 422016 Chicken
    • Entrez Gene: 8648 Human
    • Entrez Gene: 17977 Mouse
    • Entrez Gene: 574068 Pig
    • Entrez Gene: 313929 Rat
    • Omim: 602691 Human
    • SwissProt: Q15788 Human
    • SwissProt: P70365 Mouse
    • SwissProt: Q4PJW2 Pig
    • Unigene: 596314 Human
    • Unigene: 301039 Mouse
    • Unigene: 165307 Rat
    see all
  • 別名

    • bHLHe74 antibody
    • Class E basic helix-loop-helix protein 74 antibody
    • F SRC 1 antibody
    • Hin 2 protein antibody
    • Hin2 protein antibody
    • MGC129719 antibody
    • MGC129720 antibody
    • mNRC 1 antibody
    • NCoA 1 antibody
    • NCoA-1 antibody
    • Ncoa1 antibody
    • NCOA1_HUMAN antibody
    • Nuclear receptor coactivator 1 antibody
    • Nuclear receptor coactivator protein 1 antibody
    • NY REN 52 antigen antibody
    • Protein Hin 2 antibody
    • Protein Hin-2 antibody
    • Protein Hin2 antibody
    • Renal carcinoma antigen NY REN 52 antibody
    • Renal carcinoma antigen NY-REN-52 antibody
    • RIP 160 antibody
    • RIP160 antibody
    • SRC 1 antibody
    • SRC-1 antibody
    • Steroid receptor coactivator 1 antibody
    see all

画像

  • Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)
    Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)

    Immunolocalization of SRC1 in HeLa cells using ab2859.

  • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
    Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
    ab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
    Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)

    Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.

  • Flow Cytometry - Anti-KAT13A / SRC1 antibody (ab2859)
    Flow Cytometry - Anti-KAT13A / SRC1 antibody (ab2859)

    Flow cytometry analysis of SRC1 was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SRC1 Rabbit Polyclonal Antibody (PA1840, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (6)

    ab2859 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2859 は 6 報の論文で使用されています。

    • Castellaro AM  et al. Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers (Basel) 11:N/A (2019). PubMed: 30736340
    • Kong X  et al. Roux-en-Y gastric bypass enhances insulin secretion in type 2 diabetes via FXR-mediated TRPA1 expression. Mol Metab 29:1-11 (2019). PubMed: 31668381
    • Kong X  et al. FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic ß-Cells. J Diabetes Res 2019:8915818 (2019). PubMed: 31485455
    • Meerson A & Yehuda H Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells. BMC Cancer 16:882 (2016). WB ; Human . PubMed: 27842582
    • Hurtado A  et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, ChIP ; Human . PubMed: 19005469
    • Yasumoto H  et al. GNL3L inhibits activity of estrogen-related receptor gamma by competing for coactivator binding. J Cell Sci 120:2532-43 (2007). WB . PubMed: 17623774

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7 Cells)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    30 µg
    Treatment
    Vehicle, 10nm estrogen, and 1uM tamoxifen
    Specification
    MCF7 Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 23 2019

    Question

    Inquiry: Dear Sir/Madam I would like to have the price and the delivery time

    Read More

    Abcam community

    Verified customer

    Asked on Apr 23 2012

    Answer

    Thank you for contacting us.

    I am not sure if you intend to send this email to Abcam however we can offer the following products

    ab84; https://www.abcam.com/KAT13A-SRC1-antibody-1135-H4-ChIP-Grade-ab84.html

    ab2859; https://www.abcam.com/KAT13A-SRC1-antibody-ChIP-Grade-ab2859.html

    ab10308; https://www.abcam.com/KAT13A-SRC1-antibody-ab10308.html

    ab71531; https://www.abcam.com/KAT13A-SRC1-antibody-Aminoterminal-end-ab71531.html

    ab5407; https://www.abcam.com/KAT13A-SRC1-antibody-ab5407.html

    ab67101; https://www.abcam.com/KAT13A-SRC1-antibody-3532C6a-ab67101.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 23 2012

    Western blot abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (huh-7 cells)
    Loading amount
    10000 cells
    Specification
    huh-7 cells
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 11 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 37°C
    Read More

    Dr. Sungsoo Lee

    Verified customer

    投稿 May 19 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain sections)
    Specification
    brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Dr. Jiqiang Zhang

    Verified customer

    投稿 Jun 20 2007

    Immunohistochemistry (Frozen sections) abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1%
    Read More

    Dr. Jiqiang Zhang

    Verified customer

    投稿 May 23 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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