Anti-Kappa light chain 抗体 [MEM-09] (ab1050)
製品の概要
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製品名Anti-Kappa light chain antibody [MEM-09]
Kappa light chain 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [MEM-09] to Kappa light chain
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由来種Mouse
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特異性MAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM.
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アプリケーション適用あり: IP, WB, IHC-P, Flow Cyt, ICCmore details
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種交差性交差種: Human
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免疫原
Tissue/ cell preparation (Human). (Crude thymus membrane fraction).
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度>95% by SDS-PAGE
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特記事項（精製）Purified from TCS
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ポリ/モノモノクローナル
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クローン名MEM-09
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab1050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC | Use at an assay dependent concentration. |
ターゲット情報
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関連性Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
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細胞内局在Cytoplasmic and Secreted
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参照データベース
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
- Unigene: 449609 Human
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別名
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
画像
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![Western blot - Anti-Kappa light chain antibody [MEM-09] (ab1050)](https://www.abcam.co.jp/ps/products/1/ab1050/Images/ab1050_1.jpg)
Western blot - Anti-Kappa light chain antibody [MEM-09] (ab1050)This image is courtesy of an anonymous AbreviewAnti-Kappa light chain antibody [MEM-09] (ab1050) at 1/2000 dilution + Human B lymphocytes, whole cell lysate at 15 µg
Secondary
Alkaline phosphatase conjugated goat anti-mouse antibody at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 11 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 secondsThe blot was blocked with 2% milk for 2 hours at 22°C and incubated with the primary antibody for 1 hour and 20 minutes at 22°C.
プロトコール
参考文献
This product has been referenced in:
- Cao J et al. Versatile and on-demand biologics co-production in yeast. Nat Commun 9:77 (2018). Read more (PubMed: 29311542) »
- Shah KA et al. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng 112:2624-9 (2015). Read more (PubMed: 26032261) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"