Anti-Kappa light chain 抗体 [EPR5367-8] - BSA and Azide free (ab232152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5367-8] to Kappa light chain - BSA and Azide free
- Suitable for: IP, IHC-P, WB, ELISA
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free
Kappa light chain 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5367-8] to Kappa light chain - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WB, ELISAmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA
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ポジティブ・コントロール
- IP: Human spleen lysate. WB: Human tonsil, plasma and spleen lysate; IHC: Human tonsil lysate.
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特記事項
ab232152 is the carrier-free version of ab124727.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5367-8 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab232152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
細胞内局在
Cytoplasmic and Secreted -
参照データベース
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
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別名
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
画像
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This data was developed using ab124727, the same antibody clone in a different buffer formulation.
ELISA analysis of Kappa Light Chains, Lambda Light Chains, Human IgA, Human IgM, Human IgG, Rat IgG,and Mouse IgG at 1000ng/mL using purified ab124727. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/2500 dilution. -
This data was developed using ab124727, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with purified ab124727 at 1/5000 dilution (0.06 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Lane 1 : Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution (Purified)
Lanes 2-3 : Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human plasma lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 25 kDaThis data was developed using ab124727, the same antibody clone in a different buffer formulation.
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This data was developed using ab124727, the same antibody clone in a different buffer formulation.
Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
Lane 1 (input): Human spleen lysate 10µg
Lane 2 (+): ab124727 + Human spleen lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab232152 は論文での使用が確認できていません。