Anti-KAP1 (phospho S824) 抗体 [BL-246-7B5] - BSA free (ab272068)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)](https://www.abcam.co.jp/ps/products/272/ab272068/Images/ab272068-1-ab243870334271antikap1phosphos824antibodybl2467b5immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-246-7B5] to KAP1 (phospho S824) - BSA free
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free
KAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BL-246-7B5] to KAP1 (phospho S824) - BSA free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP Human
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免疫原
Synthetic peptide corresponding to Human KAP1 (phospho S824). NP_005753.1 and Gene ID 10155.
Database link: Q13263 -
特記事項
This product is sold under License from Bethyl Laboratories, Inc.
ab272068 is the carrier-free version of ab243870. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Concentration information loading... -
特記事項（精製）
Recombinant antibody was purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BL-246-7B5 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
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| IHC-P |
Human
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| IP |
Human
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. -
組織特異性
Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
ドメイン
The HP1 box is both necessary and sufficient for HP1 binding.
The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.
The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.
Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21. -
細胞内局在
Nucleus. Associated with centromeric heterochromatin during cell differentiation through CBX1. - Information by UniProt
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参照データベース
- Entrez Gene: 10155 Human
- Omim: 601742 Human
- SwissProt: Q13263 Human
- Unigene: 467408 Human
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別名
- E3 SUMO protein ligase TRIM28 antibody
- E3 SUMO-protein ligase TRIM28 antibody
- FLJ29029 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)Formalin-fixed, paraffin-embedded human breast carcinoma tissue and serial sections of etoposide treated HeLa cells stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right) are also shown. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).
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![Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)](https://www.abcam.co.jp/ps/products/272/ab272068/Images/ab272068-2-ab243870334270antikap1phosphos824antibodybl2467b5immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with etoposide (right) or untreated (left) labeling KAP-1 (phospho S824) (red) using ab243862 at 1/100 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstain: Phalloidin conjugated Alexa Fluor® 488 (green).This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)](https://www.abcam.co.jp/ps/products/272/ab272068/Images/ab272068-3-ab243870334269antikap1phosphos824antibodybl2467b5immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).
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![Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)](https://www.abcam.co.jp/ps/products/272/ab272068/Images/ab272068-4-ab243870334268antikap1phosphos824antibodybl2467b5immunoprecipitation.jpg)
Immunoprecipitation - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)KAP1 (phospho S824) was immunoprecipitated from 1 mg HEK-293T whole cell lysate treated with 100 µM etoposide or untreated, using ab243870 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243870 at 1/1000 dilution.
Lane 1: ab243870 IP in HEK-293T cell lysate treated with 100 µM etoposide.
Lane 2: ab243870 Ip in untreated HEK-293T whole cell lysate.
Lane 3: Control IgG in HEK-293T whole cell lysate treated with 100 µM etoposide.
Detection: Chemiluminescence with an exposure time of 10 seconds
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).
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![Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)](https://www.abcam.co.jp/ps/products/272/ab272068/Images/ab272068-5-benefits-of-recombinant-antibodies.png)
Anti-KAP1 (phospho S824) antibody [BL-246-7B5] - BSA free (ab272068)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272068 は論文での使用が確認できていません。