Anti-KAP1 抗体 [EPR5216] (ab109287)
![Western blot - Anti-KAP1 antibody [EPR5216] (ab109287)](https://www.abcam.co.jp/ps/products/109/ab109287/Images/ab109287-290379-anti-kap1-antibody-epr5216-western-blot.jpg "Western blot - Anti-KAP1 antibody [EPR5216] (ab109287)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5216] to KAP1
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-KAP1 antibody [EPR5216]
KAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5216] to KAP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human KAP1 protein
- IHC-P: Human colon or breast carcinoma tissue. WB: HeLa, A431, PC-3, MCF-7 cell lysates. ICC/IF: HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5216 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109287の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
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| ICC/IF | (1) |
1/100 - 1/250.
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| 特記事項 |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
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ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. -
組織特異性
Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
ドメイン
The HP1 box is both necessary and sufficient for HP1 binding.
The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.
The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.
Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21. -
細胞内局在
Nucleus. Associated with centromeric heterochromatin during cell differentiation through CBX1. - Information by UniProt
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参照データベース
- Entrez Gene: 10155 Human
- Entrez Gene: 21849 Mouse
- Entrez Gene: 116698 Rat
- Omim: 601742 Human
- SwissProt: Q13263 Human
- SwissProt: Q62318 Mouse
- SwissProt: O08629 Rat
- Unigene: 467408 Human
see all -
別名
- E3 SUMO protein ligase TRIM28 antibody
- E3 SUMO-protein ligase TRIM28 antibody
- FLJ29029 antibody
see all
画像
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Western blot - Anti-KAP1 antibody [EPR5216] (ab109287)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 + DMSO whole cell lysate (20 µg)
Lane 3: Wild type HAP1 + Blaomycin whole cell lysate (20 µg)
Lane 4: TRIM28 knockout HAP1 whole cell lysate (20 µg)
Lane 5: TRIM28 knockout HAP1 + DMSO whole cell lysate (20 µg)
Lane 6: TRIM28 knockout HAP1 + Blaomycin whole cell lysate (20 µg)
Lane 7: HeLa + DMSO whole cell lysate (20 µg)
Lane 8: HeLa + Blaomycin whole cell lysate (20 µg)Lanes 1 - 8: Merged signal (red and green). Green - ab109287 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109287 was shown to specifically react with KAP1 in wild type cells as signal was lost in KAP1 knockout cells. Wild-type and KAP1 knockout samples were subjected to SDS-PAGE. ab109287 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-KAP1 antibody [EPR5216] (ab109287)](https://www.abcam.co.jp/ps/products/109/ab109287/Images/ab109287-122026-anti-kap1-antibody-epr5216-western-blot.jpg)
Western blot - Anti-KAP1 antibody [EPR5216] (ab109287)All lanes : Anti-KAP1 antibody [EPR5216] (ab109287) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : A431 cell lysates
Lane 3 : PC-3 cell lysates
Lane 4 : MCF7 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (ab109287)](https://www.abcam.co.jp/ps/products/109/ab109287/Images/ab109287-122025-anti-kap1-antibody-epr5216-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (ab109287)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using 1/250 ab109287.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (ab109287)](https://www.abcam.co.jp/ps/products/109/ab109287/Images/ab109287-122024-anti-kap1-antibody-epr5216-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (ab109287)Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/250 ab109287.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5216] (ab109287)](https://www.abcam.co.jp/ps/products/109/ab109287/Images/ab109287-122023-anti-kap1-antibody-epr5216-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5216] (ab109287)Immunofluorescent staining of HeLa cells using 1/100 ab109287. -
![Anti-KAP1 antibody [EPR5216] (ab109287)](https://www.abcam.co.jp/ps/products/109/ab109287/Images/ab109287-6-benefits-of-recombinant-antibodies.png)
Anti-KAP1 antibody [EPR5216] (ab109287)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109287 は論文での使用が確認できていません。
