Application
Western blot
Sample
Human Cell lysate - whole cell (hES and cancer cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
hES and cancer cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 100, 90, 80 kDa
Positive control
HUES7, MCF7 and MET5A human cell lines.
Additional data
Abcam response
Thank you for sharing this data with us. ab10483 has previously been shown to work well on 293T cell lysates (please see western blot image on datasheet). However, based on this abreview ab10483 detects some non-specific proteins, under these experimental conditions. Abcam would welcome feedback from other researchers currently using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 20 2015