Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (Human embryonic kidney cells)
Total protein in input
100 µg
Specification
Human embryonic kidney cells
Immuno-precipitation step
Protein G
Other product details
Concentration
1 µg/mg lysate
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA
Additional data
Additional Notes
The ab10483 antibody was used to immunoprecipitate KAP1 from HEK293 nuclear extracts. Three different amounts of ab10483 or control IgG were tested (1, 2 or 4 ug). The eluates from these experiments as well as the supernatants from the KAP1 IPs were analyzed by Western blotting using the ab10483 antibody. As shown by Western blotting, the presence of a ~110 kDa band demonstrated that KAP1 was specifically precipitated from these extracts. The 50 and 25 kDa bands correspond to the IgG of the IP antibodies.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Seth Frietze
Verified customer
投稿 Jul 04 2011