Application
ChIP
Sample
Human Cell lysate - nuclear (Breast cancer cell line T47D)
Negative control
IgG
Specification
Breast cancer cell line T47D
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehid
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehid
Positive control
H1.2 antibody ab4086
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SDS 0.01%, triton x-100 1.1%, EDTA 1.2mM
Additional data
Abcam response
This customer received a credit to test ab22553 in ChIP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Albert Jordan
Verified customer
投稿 Apr 06 2019