Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4668] to Kaiso - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品名Anti-Kaiso antibody [EPR4668] - BSA and Azide free
Kaiso 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4668] to Kaiso - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Kaiso aa 600-700. The exact sequence is proprietary.
Ab247999 is the carrier-free version of ab124777. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247999 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 74 kDa).|
機能Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
組織特異性Expressed in vascular endothelium.
配列類似性Contains 1 BTB (POZ) domain.
Contains 3 C2H2-type zinc fingers.
細胞内局在Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.
- Information by UniProt
- Kaiso antibody
- Kaiso protein antibody
- Kaiso transcription factor antibody
All lanes : Anti-Kaiso antibody [EPR4668] (ab124777) at 1/1000 dilution
Lane 1 : Human heart lysate
Lane 2 : MCF7 lysate
Lane 3 : C6 lysate
Lane 4 : PC12 lysate
Lane 5 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
This data was developed using ab124777, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.