Anti-Kaiso 抗体 [EPR4668] - BSA and Azide free (ab247999)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4668] to Kaiso - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Kaiso antibody [EPR4668] - BSA and Azide free
Kaiso 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4668] to Kaiso - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab247999 is the carrier-free version of ab124777.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4668 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247999の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 74 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 74 kDa). |
ターゲット情報
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機能
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. -
組織特異性
Expressed in vascular endothelium. -
配列類似性
Contains 1 BTB (POZ) domain.
Contains 3 C2H2-type zinc fingers. -
細胞内局在
Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities. - Information by UniProt
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参照データベース
- Entrez Gene: 10009 Human
- Entrez Gene: 56805 Mouse
- Entrez Gene: 501506 Rat
- Omim: 300329 Human
- SwissProt: Q86T24 Human
- SwissProt: Q8BN78 Mouse
- Unigene: 143604 Human
- Unigene: 391248 Mouse
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別名
- Kaiso antibody
- Kaiso protein antibody
- Kaiso transcription factor antibody
see all
画像
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All lanes : Anti-Kaiso antibody [EPR4668] (ab124777) at 1/1000 dilution
Lane 1 : Human heart lysate
Lane 2 : MCF7 lysate
Lane 3 : C6 lysate
Lane 4 : PC12 lysate
Lane 5 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using ab124777, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247999 は論文での使用が確認できていません。