K-562 whole cell lysate (ab7911)

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製品の概要

  • 製品名

    K-562 whole cell lysate
    K562 ライゼート 製品一覧

  • 製品の詳細

    K-562 cell lysate

  • 特記事項

    Cell line: K562 (Human Chronic Myelogenous Leukemia).
    Growth media: RPMI and 10% NCS (Newborn calf serum).

     

    K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション

    適用あり: WBmore details

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Constituents: 0.77% DTT, 1% Sodium Lauryl Sulfate, 12.5% Glycerol (glycerin, glycerine), 0.01% Bromophenol blue, 0.79% Tris HCl
  • Concentration information loading...

  • ライゼート 備考

    K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50mM DTT.

  • 研究分野

  • 背景

    K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7911の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.

K562 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
特記事項
WB
Use at an assay dependent concentration.

K562 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab7911 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7911 は 1 報の論文で使用されています。

  • Baglo Y  et al. Enhanced efficacy of bleomycin in bladder cancer cells by photochemical internalization. Biomed Res Int 2014:921296 (2014). WB . PubMed: 25101299

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Question

We are currently doing western blot analysis using "Anti-FNDC5 antibody-Carboxyterminal end (ab93373)" from abcam. As its datasheet mentions, K562 cell line lysate was used as positive control, which we are planning to orderas soon as possible. However, we are not sure whether we should order "K562 whole cell lysate-tumor cell line (ab29306)" or "k562 whole cell lysate (ab7911)" for this purpose. Moreover, could you please kindly email me the western blot condition when using this lysate?

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Answer

Thank you for contacting us.

The lysates ab29306 and ab7911 were prepared using slightly different techniques, but will give equivalent results in WB. Either of these lysates will be guaranteed for use as a positive control with ab93373. We recommendloading 20ug of lysate per lane. Ab29306should be boiled for 5 minutes prior to loading in thegel, but ab7911 has already beenboiled.I hope this helps, please let me know if you need any additional information or assistance.

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Question

What is the cell concentration (cells/mL) of the lysate?

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Answer

Thanks for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry. The lysate is vialed in terms of protein concentration, so unfortunately the lab does not have any information about the cell concentration. I am sorry we don't have the information you are looking for, but if there is anything else that we can help you with please let me know.

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