製品名Jurkat nuclear extract lysate (TPA + CI stimulated)
Jurkat ライゼート 製品一覧
特記事項Cell line: Jurkat (Human T cell lymphoblast-like).
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.
保存方法Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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背景Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.