Anti-Junctional Adhesion Molecule 1/JAM-A 抗体 [EPR23244-12] (ab269948)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23244-12] to Junctional Adhesion Molecule 1/JAM-A
- Suitable for: ICC/IF, WB, Flow Cyt, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12]
Junctional Adhesion Molecule 1/JAM-A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23244-12] to Junctional Adhesion Molecule 1/JAM-A -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, Flow Cyt, IPmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human lung tissue lysate. HT-29, HUVEC and TF-1 whole cell lysate. ICC/IF: HT-29 cells. Flow Cyt: HT-29 cells, human B lymphocytes and human monocytes. IP: HT-29 whole cell lysate. Human lung tissue lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23244-12 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab269948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/50.
|
|
WB |
1/1000. Predicted molecular weight: 32 kDa.
|
|
Flow Cyt |
1/500.
|
|
IP |
1/30.
|
特記事項 |
---|
ICC/IF
1/50. |
WB
1/1000. Predicted molecular weight: 32 kDa. |
Flow Cyt
1/500. |
IP
1/30. |
ターゲット情報
-
機能
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. -
配列類似性
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells. - Information by UniProt
-
参照データベース
- Entrez Gene: 50848 Human
- Omim: 605721 Human
- SwissProt: Q9Y624 Human
- Unigene: 517293 Human
-
別名
- CD 321 antibody
- CD321 antibody
- CD321 antigen antibody
see all
画像
-
All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] (ab269948) at 1/1000 dilution
Lane 1 : Human lung lysate
Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 4 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 5 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
Lane 6 : TF-1 (human erythroleukemia erythroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10698320, 10753840).
Low expression control: THP-1 and Molt-4 (PMID:10698320).
Exposure time: Lanes 1-5: 15 seconds; Lane 6:70 seconds.
-
Junctional Adhesion Molecule 1/JAM-A was immunoprecipitated from 0.35 mg human lung lysate 10µg with ab269948 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab269948 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Human lung lysate 10µg.
Lane 2: ab269948 IP in human lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269948 in human lung lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
-
Junctional Adhesion Molecule 1/JAM-A was immunoprecipitated from 0.35 mg HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg with ab269948 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab269948 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: HT-29 whole cell lysate 10µg.
Lane 2: ab269948 IP in HT-29 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269948 in HT-29 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
-
Flow cytometric analysis of human B lymphocytes (Left) / human monocytes (Right) cells labeling Junctional Adhesion Molecule 1/JAM-A with ab269948 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Human peripheral blood mononuclear cell (PBMC) co-stained with anti-CD19 conjugated to PE-Cy7 and anti-CD14 conjugated to BV510. JAM-A expression on B lymphocytes (CD19+) and monocytes (CD14+) population are shown respectively.
Gated on viable cells.
-
Flow cytometric analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Junctional Adhesion Molecule 1/JAM-A with ab269948 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Gated on viable cells.
-
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma epithelial cell) cells labeling Junctional Adhesion Molecule 1/JAM-A with ab269948 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HT-29 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab269948 は 1 報の論文で使用されています。
- Deng L et al. Bioengineered miR-124-3p prodrug selectively alters the proteome of human carcinoma cells to control multiple cellular components and lung metastasis in vivo. Acta Pharm Sin B 11:3950-3965 (2021). PubMed: 35024318