製品名Anti-Junctional Adhesion Molecule 1/JAM-A antibody
Junctional Adhesion Molecule 1/JAM-A 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Junctional Adhesion Molecule 1/JAM-A
アプリケーション適用あり: WBmore details
種交差性交差種: Mouse, Rat
交差が予測される動物種: Chinese hamster
Synthetic peptide corresponding to Mouse Junctional Adhesion Molecule 1/JAM-A aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex; Mouse Cerebellum; Mouse Spinal Cord.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab125886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).|
機能Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
配列類似性Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
細胞内局在Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
- Information by UniProt
- CD 321 antibody
- CD321 antibody
- CD321 antigen antibody
All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Rat Cortex Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa, 99 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Junctional Adhesion Molecule 1/JAM-A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1/JAM-A is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab125886 has not yet been referenced specifically in any publications.