製品の概要

  • 製品名
    Anti-JunB antibody [EPR6518]
    JunB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6518] to JunB
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, ICC/IFmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human JunB (N terminal). The exact sequence is proprietary.
    Database link: P17275

  • ポジティブ・コントロール
    • WB: MCF7, Human tonsil, Human uterus cancer, mouse uterus, rat lung, rat spleen, HeLa and HACAT lysates ICC/IF: HeLa, and MCF7 cells. IP: HeLa cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab128878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200. Predicted molecular weight: 36 kDa.

For unpurified use at 1/1000 - 1/10000.

IP 1/10 - 1/100.
ICC/IF 1/200.

For unpurified use at 1/250- 1/500.

  • 追加情報
    Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    画像

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1:200 dilution (9.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 �g/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 �g/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/200 dilution (Purified)

      Lane 1 : Rat lung lysates
      Lane 2 : Rat spleen lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse uterus lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?

    • Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified) + Human uterus lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?

    • ab128878 (purified) at 1:100 dilution (2 µg) immunoprecipitating JunB in HeLa whole cell lysates.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
      Lane 2 (+): ab128878 & HeLa whole cell lysates
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP)�(ab131366) was used at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Unpurified)

      Lane 1 : MCF-7 lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : Human uterus cancer lysate
      Lane 4 : HeLa lysate
      Lane 5 : HACAT lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • Unpurified ab128878, at a 1/250 dilution, staining JunB in MCF7 (Human breast adenocarcinoma cell line) cells by Immunofluorescence.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:
    • Shirali AS  et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). Read more (PubMed: 29615716) »
    • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »
    See all 3 Publications for this product

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