Anti-JunB 抗体 (ab3525)

Submit a review Q&A (1)References (2)
Western blot - Anti-JunB antibody (ab3525)

    Key features and details

    • Rabbit polyclonal to JunB
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-JunB antibody
      JunB 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to JunB

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse
      交差が予測される動物種: Rat, Cow, Human

    • 免疫原

      Synthetic peptide corresponding to Mouse JunB aa 45-61.
      Sequence:

      DPYRGLKGPGARGPGPE


      Database link: P09450
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • WB: uninduced and induced L929 cells.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab3525 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

    ターゲット情報

    画像

    • Western blot - Anti-JunB antibody (ab3525)
      Western blot - Anti-JunB antibody (ab3525)
      All lanes : Anti-JunB antibody (ab3525)

      Lane 1 : L929 cell extract
      Lane 2 : Extract from L929 cells treated with dexamethasone

      Predicted band size: 38 kDa
      Observed band size: 38 kDa
      Additional bands at: 50 kDa (possible cross reactivity)



      ab3525 in Western blot on L929 cells.  There is the specific band at 38 kDa.  There are also non-specific bands at 50 kDa and a very faint one at 40 kDa.

    参考文献 (2)

    ab3525 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3525 は 2 報の論文で使用されています。

    • Pang Q  et al. Tooth loss, cognitive impairment and chronic cerebral ischemia. J Dent Sci 15:84-91 (2020). PubMed: 32257004
    • Liu B  et al. Paeoniflorin Inhibits Mesangial Cell Proliferation and Inflammatory Response in Rats With Mesangial Proliferative Glomerulonephritis Through PI3K/AKT/GSK-3ß Pathway. Front Pharmacol 10:978 (2019). PubMed: 31551783

    レビューと Q&A

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    Question

    Dear Sir/Madam, I was just wondering if you have a picture of sample Western blot with anti-JunB antibodies. The reason for my curiosity is that I already have anti-JunB from the Santa Cruz, but the specificity of this product is below my expectations. Thank you very much in advance.

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    Answer

    I have an image of this antibody in Western blot, which I have added onto the datasheet, on L929 cells. There is the specific band at 38 kDa. There are also non-specific bands at 50 kDa and a very faint one at 40 kDa. Please refer to the online datasheet for this image.

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