Anti-JunB 抗体 (ab3525)

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Key features and details

  • Rabbit polyclonal to JunB
  • Suitable for: WB, ChIP, IP
  • Reacts with: Mouse
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-JunB antibody
    JunB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to JunB

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ChIP, IPmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Rat, Cow, Human

  • 免疫原

    Synthetic peptide corresponding to Mouse JunB aa 45-61.
    Sequence:

    DPYRGLKGPGARGPGPE


    Database link: P09450

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: uninduced and induced L929 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ChIP Use at an assay dependent concentration.

Dilution = 1-3 ul
IP Use at an assay dependent concentration.

Dilution: assay dependent

ターゲット情報

画像

  • Western blot - Anti-JunB antibody (ab3525)
    Western blot - Anti-JunB antibody (ab3525)
    All lanes : Anti-JunB antibody (ab3525)

    Lane 1 : L929 cell extract
    Lane 2 : Extract from L929 cells treated with dexamethasone

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 50 kDa (possible cross reactivity)



    ab3525 in Western blot on L929 cells.  There is the specific band at 38 kDa.  There are also non-specific bands at 50 kDa and a very faint one at 40 kDa.

参考文献 (1)

ab3525 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3525 は 1 報の論文で使用されています。

  • Liu B  et al. Paeoniflorin Inhibits Mesangial Cell Proliferation and Inflammatory Response in Rats With Mesangial Proliferative Glomerulonephritis Through PI3K/AKT/GSK-3ß Pathway. Front Pharmacol 10:978 (2019). PubMed: 31551783

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Question

Dear Sir/Madam, I was just wondering if you have a picture of sample Western blot with anti-JunB antibodies. The reason for my curiosity is that I already have anti-JunB from the Santa Cruz, but the specificity of this product is below my expectations. Thank you very much in advance.

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Answer

I have an image of this antibody in Western blot, which I have added onto the datasheet, on L929 cells. There is the specific band at 38 kDa. There are also non-specific bands at 50 kDa and a very faint one at 40 kDa. Please refer to the online datasheet for this image.

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