Anti-JunB 抗体 (ab3525)
Key features and details
- Rabbit polyclonal to JunB
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-JunB antibody
JunB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to JunB -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow -
免疫原
Synthetic peptide corresponding to Mouse JunB aa 1-100.
Database link: P09450 -
ポジティブ・コントロール
- WB: uninduced and induced L929 cells. ICC/IF: PC-3 (Human prostate adenocarcinoma cell line)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: 100% PBS -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3525の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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ICC/IF |
Use a concentration of 2 µg/ml.
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特記事項 |
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WB
Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. -
配列類似性
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain. -
翻訳後修飾
Ubiquitinated by ITCH, leading to its degradation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 514246 Cow
- Entrez Gene: 3726 Human
- Entrez Gene: 16477 Mouse
- Entrez Gene: 24517 Rat
- Omim: 165161 Human
- SwissProt: Q0VBZ5 Cow
- SwissProt: P17275 Human
- SwissProt: P09450 Mouse
see all -
別名
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
画像
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All lanes : Anti-JunB antibody (ab3525)
Lane 1 : L929 cell extract
Lane 2 : Extract from L929 cells treated with dexamethasone
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 50 kDa (possible cross reactivity)
ab3525 in Western blot on L929 cells. There is the specific band at 38 kDa. There are also non-specific bands at 50 kDa and a very faint one at 40 kDa. -
Immunofluorescent analysis of PC-3 (Human prostate adenocarcinoma cell line) cells on 70% confluent log phase labeling JunB. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3525 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab3525 は 3 報の論文で使用されています。
- Pang Q et al. Tooth loss, cognitive impairment and chronic cerebral ischemia. J Dent Sci 15:84-91 (2020). PubMed: 32257004
- Halbgebauer D et al. Browning capabilities of human primary adipose-derived stromal cells compared to SGBS cells. Sci Rep 10:9632 (2020). PubMed: 32541826
- Liu B et al. Paeoniflorin Inhibits Mesangial Cell Proliferation and Inflammatory Response in Rats With Mesangial Proliferative Glomerulonephritis Through PI3K/AKT/GSK-3ß Pathway. Front Pharmacol 10:978 (2019). PubMed: 31551783