Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Treatment
150uM anisomycin for 30mins
Specification
HEK293
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
LI-COR
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 40, 50 approx kDa Non-specific: 25, 28, 35 approx kDa
Additional data
Additional Notes
lane 1 untreated control
lane 2 treated anisomycin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 01 2008