Anti-JLP 抗体 (ab12331)
Key features and details
- Rabbit polyclonal to JLP
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-JLP antibody
JLP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to JLP -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
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免疫原
Synthetic peptide within Human JLP aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60271 -
ポジティブ･コントロール
- IP: HeLa nuclear extract. WB: HeLa, 293T,Jurkat, 3T3 cell lysates IHC-P: Human testis, and seminoma tissues
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody is affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12331の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
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| IP |
Use a concentration of 2 - 5 µg/ml.
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| 特記事項 |
|---|
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa. |
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IP
Use a concentration of 2 - 5 µg/ml. |
ターゲット情報
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関連性
JLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. -
細胞内局在
Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. -
参照データベース
- Entrez Gene: 9043 Human
- Entrez Gene: 70834 Mouse
- Omim: 605430 Human
- SwissProt: O60271 Human
- SwissProt: Q58A65 Mouse
- SwissProt: Q8CJC2 Mouse
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別名
- C jun amino terminal kinase interacting protein 4 antibody
- c Jun NH2 terminal kinase associated leucine zipper protein antibody
- FLJ13450 antibody
see all
画像
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Western blot - Anti-JLP antibody (ab12331)All lanes : Anti-JLP antibody (ab12331) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 156.8 kDa -
Immunoprecipitation - Anti-JLP antibody (ab12331)Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). ab12331 was used for immunoprecipitation at 3 µg/mg lysate and used for western blot at 1 µg/ml. Lanes 1 and 2 - ab12331 IP, Lane 3 - control IgG.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12331)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human seminoma using ab12331 at 1/1000 with and HRP- conjugated goat anti-rabbit secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab12331 は 17 報の論文で使用されています。
- Yan Q et al. A negative feedback loop between JNK-associated leucine zipper protein and TGF-ß1 regulates kidney fibrosis. Commun Biol 3:288 (2020). PubMed: 32504044
- Yan P et al. LncRNA NEAT1 enhances the resistance of anaplastic thyroid carcinoma cells to cisplatin by sponging miR-9-5p and regulating SPAG9 expression. Int J Oncol 55:988-1002 (2019). PubMed: 31485599
- Luo S et al. SPAG9/MKK3/p38 axis is a novel therapeutic target for liver cancer. Oncol Rep 41:2329-2336 (2019). PubMed: 30720101
- Ren B et al. Sperm-associated antigen 9 is upregulated in hepatocellular carcinoma tissue and enhances QGY cell proliferation and invasion in vitro. Oncol Lett 15:415-422 (2018). PubMed: 29391885
- Fan W et al. Hsp70 Interacts with Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinase 2 To Regulate p38MAPK Stability and Myoblast Differentiation during Skeletal Muscle Regeneration. Mol Cell Biol 38:N/A (2018). PubMed: 30275345