Application
Flow Cytometry
Sample
Mouse Cell (Skin)
Permeabilization
No
Specification
Skin
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/100
Secondary antibody
Dilution
1/600
Name
Non-Abcam antibody was used: anti rabbit Alexa Flour 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
This antibody produced a good signal on mouse epidermal cells by flow cytometry based on the isotype control. However, we still got a large amount of antibody staining on the Jagged1 knockout epidermal cells- indicating that the antibody has a lot of background staining in our samples.
Additional Info:
The cells were knocked out utilizing a Cre-flox system. This was confirmed with PCR analysis.
Abcam response
Thank you for testing this antibody in Flow Cytometry. It may be that ab7771 antibody is not suitable for this application. We appreciate your feedback and will continue to gather data on its use in Flow.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 20 2015